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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- HPA028487 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA028487, RRID:AB_10602219
- Product name
- Anti-EIF4G1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human EIF4G1, Gene description: eukaryotic translation initiation factor 4 gamma, 1, Alternative Gene Names: EIF4F, EIF4G, p220, PARK18, Validated applications: ICC, IHC, WB, Uniprot ID: Q04637, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Colocalization of TDP‐43 and stress granules at the early stage of TDP‐43 aggregation in amyotrophic lateral sclerosis
A Novel HOXA10-Associated 5-Gene–Based Prognostic Signature for Stratification of Short-term Survivors of Pancreatic Ductal Adenocarcinoma
Mori F, Yasui H, Miki Y, Kon T, Arai A, Kurotaki H, Tomiyama M, Wakabayashi K
Brain Pathology 2023;34(2)
Brain Pathology 2023;34(2)
A Novel HOXA10-Associated 5-Gene–Based Prognostic Signature for Stratification of Short-term Survivors of Pancreatic Ductal Adenocarcinoma
Kisling S, Atri P, Shah A, Cox J, Sharma S, Smith L, Ghersi D, Batra S
Clinical Cancer Research 2023;29(18):3759-3770
Clinical Cancer Research 2023;29(18):3759-3770
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-EIF4G1 antibody. Remaining relative intensity is presented.
- Sample type
- Human
- Protocol
- Protocol
Enhanced validation
- Submitted by
- 55af80e3e0991
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Confocal images of immunofluorescently stained human U-2 OS cells.The protein EIF4G1 is shown in green. The image to the left show cells transfected with control siRNA and the image to the right show cells where EIF4G1 has been downregulated with specific siRNA.
- Sample type
- U-2 OS cells
- Primary Ab dilution
- 1:23
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:800
- Knockdown/Genetic Approaches Application
- Immunocytochemistry
