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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS64046 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- EIF4G1 (phospho S1148) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to residues in Human EIF4G1 around the phosphorylation site of S1148.
- Description
- The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA ¡ùcap¡ì during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A, and the eIF3 complex .
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of p-EIF4G1(S1148) pAb at 1:500 dilution Lane1:THP-1 treated with EGF(0.1ng/m) 30 min whole cell lysateLane2:PC12 treated with EGF(0.1ng/m) 30 min whole cell lysateLane3:Raw264.7 treated with EGF(0.1ng/m) 30 min whole cell lysate
