TA322698

antibody from OriGene

Targeting: EIF4G1 EIF4F, EIF4G, p220, PARK18

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA322698

Provider

OriGene

Product name

Anti-EIF4G1 (phospho-Ser1232) Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Anti-EIF4G1 (phospho-Ser1232) Rabbit Polyclonal Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

EIF4G1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 220 kDa . Positive control: HT29 cells untreated or treated with Anisomycin lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: HT29 cells untreated with Anisomycin lysate Lane2: HT29 cells treated with Anisomycin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm. Positive control: Human lung carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human lung carcinoma tissue using EIF4G1 (phospho-Ser1232) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

Validation comment

IHC