Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00001981-M10 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001981-M10, RRID:AB_828846
- Product name
- Mouse Monoclonal eIF4G1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. EIF4G1 (2A9)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in transfected 293T cell line by EIF4G1 monoclonal antibody (M10), clone 2A9. Lane 1: EIF4G1 transfected lysatE (70.95 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in PC-12 (Cat # L012V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: eIF4G1 Antibody (2A9) [H00001981-M10] - Detection limit for recombinant GST tagged EIF4G1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of monoclonal antibody to EIF4G1 on HeLa cell. Antibody concentration 10 ug/ml