15704-1-AP

antibody from Invitrogen Antibodies

Targeting: EIF4G1 EIF4F, EIF4G, p220, PARK18

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15704-1-AP

Provider

Invitrogen Antibodies

Product name

EIF4G1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: IEEYLHLND MKEAVQCVQE LASPSLLFIF VRHGVESTLE RSAIAREHMG QLLHQLLCAG HLSTAQYYQG LYEILELAED MEIDIPHVWL YLAELVTPIL QEGGVPMGEL FREITKPLRP LGKAASLLLE ILGLLCKSMG PKKVGTLWRE AGLSWKEFLP EGQDIGAFVA EQKVEYTLGE ESEAPGQRAL PSEELNRQLE KLLKEGSSNQ RVFDWIEANL SEQQIVSNTL VRALMTAVCY SAIIFETPLR VDVAVLKARA KLLQKYLCDE QKELQALYAL QALVVTLEQP PNLLRMFFDA LYDEDVVKED AFYSWESSKD PAEQQGKGVA LKSVTAFFKW LREAEEESDH N (296-645 aa encoded by BC007788)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.23 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using EIF4G1 antibody 15704-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 15704-1-AP (EIF4G1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 15704-1-AP (EIF4G1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15704-1-AP (EIF4G1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15704-1-AP (EIF4G1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-EIF4G1 (IP:15704-1-AP, 5ug; Detection:15704-1-AP 1:500) with HeLa cells lysate 2000ug.