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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [25]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-268 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-268, RRID:AB_10001835
- Product name
- Rabbit Polyclonal eIF4G1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Leucine supplementation stimulates protein synthesis and reduces degradation signal activation in muscle of newborn pigs during acute endotoxemia.
Anabolic signaling and protein deposition are enhanced by intermittent compared with continuous feeding in skeletal muscle of neonates.
A cellular response linking eIF4AI activity to eIF4AII transcription.
Reversing chemoresistance by small molecule inhibition of the translation initiation complex eIF4F.
Intermittent bolus feeding has a greater stimulatory effect on protein synthesis in skeletal muscle than continuous feeding in neonatal pigs.
Dysregulation of mTOR signaling in fragile X syndrome.
The adenovirus E1B 55-kilodalton and E4 open reading frame 6 proteins limit phosphorylation of eIF2alpha during the late phase of infection.
Feeding rapidly stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing translation initiation.
Effects of chronic overload on muscle hypertrophy and mTOR signaling in young adult and aged rats.
Fed levels of amino acids are required for the somatotropin-induced increase in muscle protein synthesis.
Leucine stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing mTORC1 activation.
Stimulation of muscle protein synthesis by somatotropin in pigs is independent of the somatotropin-induced increase in circulating insulin.
c-Myc and eIF4F are components of a feedforward loop that links transcription and translation.
ERK and mTOR signaling couple beta-adrenergic receptors to translation initiation machinery to gate induction of protein synthesis-dependent long-term potentiation.
Steroid and oxygen effects on eIF4F complex, mTOR, and ENaC translation in fetal lung epithelia.
Rapamycin blunts nutrient stimulation of eIF4G, but not PKCepsilon phosphorylation, in skeletal muscle.
Amino acid availability and age affect the leucine stimulation of protein synthesis and eIF4F formation in muscle.
Post-transcriptional regulation of thioredoxin by the stress inducible heterogenous ribonucleoprotein A18.
Regulation of eukaryotic initiation factor 4E by converging signaling pathways during metabotropic glutamate receptor-dependent long-term depression.
RNA-mediated sequestration of the RNA helicase eIF4A by Pateamine A inhibits translation initiation.
Age-associated decrease in contraction-induced activation of downstream targets of Akt/mTor signaling in skeletal muscle.
Meal feeding enhances formation of eIF4F in skeletal muscle: role of increased eIF4E availability and eIF4G phosphorylation.
Effects of dietary carbohydrate restriction with high protein intake on protein metabolism and the somatotropic axis.
Acute alcohol intoxication enhances myocardial eIF4G phosphorylation despite reducing mTOR signaling.
IGF-I/IGFBP-3 ameliorates alterations in protein synthesis, eIF4E availability, and myostatin in alcohol-fed rats.
Hernandez-García AD, Columbus DA, Manjarín R, Nguyen HV, Suryawan A, Orellana RA, Davis TA
American journal of physiology. Endocrinology and metabolism 2016 Oct 1;311(4):E791-E801
American journal of physiology. Endocrinology and metabolism 2016 Oct 1;311(4):E791-E801
Anabolic signaling and protein deposition are enhanced by intermittent compared with continuous feeding in skeletal muscle of neonates.
El-Kadi SW, Suryawan A, Gazzaneo MC, Srivastava N, Orellana RA, Nguyen HV, Lobley GE, Davis TA
American journal of physiology. Endocrinology and metabolism 2012 Mar 15;302(6):E674-86
American journal of physiology. Endocrinology and metabolism 2012 Mar 15;302(6):E674-86
A cellular response linking eIF4AI activity to eIF4AII transcription.
Galicia-Vázquez G, Cencic R, Robert F, Agenor AQ, Pelletier J
RNA (New York, N.Y.) 2012 Jul;18(7):1373-84
RNA (New York, N.Y.) 2012 Jul;18(7):1373-84
Reversing chemoresistance by small molecule inhibition of the translation initiation complex eIF4F.
Cencic R, Hall DR, Robert F, Du Y, Min J, Li L, Qui M, Lewis I, Kurtkaya S, Dingledine R, Fu H, Kozakov D, Vajda S, Pelletier J
Proceedings of the National Academy of Sciences of the United States of America 2011 Jan 18;108(3):1046-51
Proceedings of the National Academy of Sciences of the United States of America 2011 Jan 18;108(3):1046-51
Intermittent bolus feeding has a greater stimulatory effect on protein synthesis in skeletal muscle than continuous feeding in neonatal pigs.
Gazzaneo MC, Suryawan A, Orellana RA, Torrazza RM, El-Kadi SW, Wilson FA, Kimball SR, Srivastava N, Nguyen HV, Fiorotto ML, Davis TA
The Journal of nutrition 2011 Dec;141(12):2152-8
The Journal of nutrition 2011 Dec;141(12):2152-8
Dysregulation of mTOR signaling in fragile X syndrome.
Sharma A, Hoeffer CA, Takayasu Y, Miyawaki T, McBride SM, Klann E, Zukin RS
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Jan 13;30(2):694-702
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Jan 13;30(2):694-702
The adenovirus E1B 55-kilodalton and E4 open reading frame 6 proteins limit phosphorylation of eIF2alpha during the late phase of infection.
Spurgeon ME, Ornelles DA
Journal of virology 2009 Oct;83(19):9970-82
Journal of virology 2009 Oct;83(19):9970-82
Feeding rapidly stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing translation initiation.
Wilson FA, Suryawan A, Orellana RA, Kimball SR, Gazzaneo MC, Nguyen HV, Fiorotto ML, Davis TA
The Journal of nutrition 2009 Oct;139(10):1873-80
The Journal of nutrition 2009 Oct;139(10):1873-80
Effects of chronic overload on muscle hypertrophy and mTOR signaling in young adult and aged rats.
Chalé-Rush A, Morris EP, Kendall TL, Brooks NE, Fielding RA
The journals of gerontology. Series A, Biological sciences and medical sciences 2009 Dec;64(12):1232-9
The journals of gerontology. Series A, Biological sciences and medical sciences 2009 Dec;64(12):1232-9
Fed levels of amino acids are required for the somatotropin-induced increase in muscle protein synthesis.
Wilson FA, Suryawan A, Orellana RA, Nguyen HV, Jeyapalan AS, Gazzaneo MC, Davis TA
American journal of physiology. Endocrinology and metabolism 2008 Oct;295(4):E876-83
American journal of physiology. Endocrinology and metabolism 2008 Oct;295(4):E876-83
Leucine stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing mTORC1 activation.
Suryawan A, Jeyapalan AS, Orellana RA, Wilson FA, Nguyen HV, Davis TA
American journal of physiology. Endocrinology and metabolism 2008 Oct;295(4):E868-75
American journal of physiology. Endocrinology and metabolism 2008 Oct;295(4):E868-75
Stimulation of muscle protein synthesis by somatotropin in pigs is independent of the somatotropin-induced increase in circulating insulin.
Wilson FA, Orellana RA, Suryawan A, Nguyen HV, Jeyapalan AS, Frank J, Davis TA
American journal of physiology. Endocrinology and metabolism 2008 Jul;295(1):E187-94
American journal of physiology. Endocrinology and metabolism 2008 Jul;295(1):E187-94
c-Myc and eIF4F are components of a feedforward loop that links transcription and translation.
Lin CJ, Cencic R, Mills JR, Robert F, Pelletier J
Cancer research 2008 Jul 1;68(13):5326-34
Cancer research 2008 Jul 1;68(13):5326-34
ERK and mTOR signaling couple beta-adrenergic receptors to translation initiation machinery to gate induction of protein synthesis-dependent long-term potentiation.
Gelinas JN, Banko JL, Hou L, Sonenberg N, Weeber EJ, Klann E, Nguyen PV
The Journal of biological chemistry 2007 Sep 14;282(37):27527-35
The Journal of biological chemistry 2007 Sep 14;282(37):27527-35
Steroid and oxygen effects on eIF4F complex, mTOR, and ENaC translation in fetal lung epithelia.
Otulakowski G, Duan W, Gandhi S, O'brodovich H
American journal of respiratory cell and molecular biology 2007 Oct;37(4):457-66
American journal of respiratory cell and molecular biology 2007 Oct;37(4):457-66
Rapamycin blunts nutrient stimulation of eIF4G, but not PKCepsilon phosphorylation, in skeletal muscle.
Vary TC, Anthony JC, Jefferson LS, Kimball SR, Lynch CJ
American journal of physiology. Endocrinology and metabolism 2007 Jul;293(1):E188-96
American journal of physiology. Endocrinology and metabolism 2007 Jul;293(1):E188-96
Amino acid availability and age affect the leucine stimulation of protein synthesis and eIF4F formation in muscle.
Escobar J, Frank JW, Suryawan A, Nguyen HV, Davis TA
American journal of physiology. Endocrinology and metabolism 2007 Dec;293(6):E1615-21
American journal of physiology. Endocrinology and metabolism 2007 Dec;293(6):E1615-21
Post-transcriptional regulation of thioredoxin by the stress inducible heterogenous ribonucleoprotein A18.
Yang R, Weber DJ, Carrier F
Nucleic acids research 2006;34(4):1224-36
Nucleic acids research 2006;34(4):1224-36
Regulation of eukaryotic initiation factor 4E by converging signaling pathways during metabotropic glutamate receptor-dependent long-term depression.
Banko JL, Hou L, Poulin F, Sonenberg N, Klann E
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Feb 22;26(8):2167-73
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Feb 22;26(8):2167-73
RNA-mediated sequestration of the RNA helicase eIF4A by Pateamine A inhibits translation initiation.
Bordeleau ME, Cencic R, Lindqvist L, Oberer M, Northcote P, Wagner G, Pelletier J
Chemistry & biology 2006 Dec;13(12):1287-95
Chemistry & biology 2006 Dec;13(12):1287-95
Age-associated decrease in contraction-induced activation of downstream targets of Akt/mTor signaling in skeletal muscle.
Funai K, Parkington JD, Carambula S, Fielding RA
American journal of physiology. Regulatory, integrative and comparative physiology 2006 Apr;290(4):R1080-6
American journal of physiology. Regulatory, integrative and comparative physiology 2006 Apr;290(4):R1080-6
Meal feeding enhances formation of eIF4F in skeletal muscle: role of increased eIF4E availability and eIF4G phosphorylation.
Vary TC, Lynch CJ
American journal of physiology. Endocrinology and metabolism 2006 Apr;290(4):E631-42
American journal of physiology. Endocrinology and metabolism 2006 Apr;290(4):E631-42
Effects of dietary carbohydrate restriction with high protein intake on protein metabolism and the somatotropic axis.
Harber MP, Schenk S, Barkan AL, Horowitz JF
The Journal of clinical endocrinology and metabolism 2005 Sep;90(9):5175-81
The Journal of clinical endocrinology and metabolism 2005 Sep;90(9):5175-81
Acute alcohol intoxication enhances myocardial eIF4G phosphorylation despite reducing mTOR signaling.
Vary TC, Deiter G, Goodman SA
American journal of physiology. Heart and circulatory physiology 2005 Jan;288(1):H121-8
American journal of physiology. Heart and circulatory physiology 2005 Jan;288(1):H121-8
IGF-I/IGFBP-3 ameliorates alterations in protein synthesis, eIF4E availability, and myostatin in alcohol-fed rats.
Lang CH, Frost RA, Svanberg E, Vary TC
American journal of physiology. Endocrinology and metabolism 2004 Jun;286(6):E916-26
American journal of physiology. Endocrinology and metabolism 2004 Jun;286(6):E916-26
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4G1 Antibody [NB100-268] - Rat liver lysate [10K x g supernatant, ~50 ug (left) or 150 ug (right lane)] separated on 7.5% gel and transferred to PVDF membranes.Antibody: Affinity purified rabbit anti-eIF4G used at the indicated concentrations. Detection:Chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4G1 Antibody [NB100-268] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: eIF4G1 Antibody [NB100-268] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for IP at 6 ug per reaction. eiF4G1/eiF4GI was also immunoprecipitated by rabbit antieiF4G1/eiF4GI antibody. For blotting immunoprecipitated eiF4G1/eiF4GI, rabbit antieiF4G1/eiF4GI antibody was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
