MA5-16749

antibody from Invitrogen Antibodies

Targeting: IL10 CSIF, IL-10, IL10A, TGIF

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Flow cytometry (Recommended by provider)

Other assay (Recommended by provider)

Antibody Data

Product number

MA5-16749

Provider

Invitrogen Antibodies

Product name

IL-10 Monoclonal Antibody (JES3-9D7)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL. This product is a low-endotoxin, preservative-free formulation. Endotoxin level is

Reactivity

Human

Host

Rat

Isotype

IgG

Antibody clone number

JES3-9D7

Vial size

500 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Optimization of mass cytometry sample cryopreservation after staining.

Sumatoh HR, Teng KW, Cheng Y, Newell EW
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2017 Jan;91(1):48-61

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Mucosal Inducible NO Synthase-Producing IgA+ Plasma Cells in Helicobacter pylori-Infected Patients.

Neumann L, Mueller M, Moos V, Heller F, Meyer TF, Loddenkemper C, Bojarski C, Fehlings M, Doerner T, Allers K, Aebischer T, Ignatius R, Schneider T
Journal of immunology (Baltimore, Md. : 1950) 2016 Sep 1;197(5):1801-8

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Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.

Miles B, Miller SM, Folkvord JM, Levy DN, Rakasz EG, Skinner PJ, Connick E
PLoS pathogens 2016 Oct;12(10):e1005924

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Effects of B Cell Depletion on Early Mycobacterium tuberculosis Infection in Cynomolgus Macaques.

Phuah J, Wong EA, Gideon HP, Maiello P, Coleman MT, Hendricks MR, Ruden R, Cirrincione LR, Chan J, Lin PL, Flynn JL
Infection and immunity 2016 May;84(5):1301-1311

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Systemic and compartmentalised immune responses in a Leishmania braziliensis-macaque model of self-healing cutaneous leishmaniasis.

de-Campos SN, Souza-Lemos C, Teva A, Porrozzi R, Grimaldi G Jr
Veterinary immunology and immunopathology 2010 Sep 15;137(1-2):149-54

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Application of six-color flow cytometry for the assessment of dendritic cell responses in whole blood assays.

Della Bella S, Giannelli S, Taddeo A, Presicce P, Villa ML
Journal of immunological methods 2008 Dec 31;339(2):153-64

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A soluble factor produced by lamina propria mononuclear cells is required for TNF-alpha enhancement of IFN-gamma production by T cells.

Prehn JL, Landers CJ, Targan SR
Journal of immunology (Baltimore, Md. : 1950) 1999 Oct 15;163(8):4277-83

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-IL-10 Monoclonal Antibody (JES3-9D7) (Product # MA5-16749) and a 18 kDa band corresponding to Interleukin-10 was observed in Human rec. IL10 as compared to Human rec. IL6 which was used as a negative control. Mouse rec. IL10 was used to determine species cross reactivity. 250 ng of Human rec. IL10 (Lane 1), Human rec. IL6 (Lane 2), Mouse rec. IL10 (Lane 3) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with F(ab2-Rabbit anti-Rat IgG (H+L Secondary Antibody, HRP (Product # PA1-29927,1:20000 dilution) using the iBright FL 1500 (Product # A44115). Chemiluminescentdetection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).