AB-217-NA

antibody from Novus Biologicals

Targeting: IL10 CSIF, IL-10, IL10A, TGIF

Western blot (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AB-217-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal IL-10 Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects human IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant equine IL-10, recombinant canine IL-10, recombinant porcine IL-10, and recombinant feline IL-10 is observed, and less than 10% cross-reactivity with recombinant mouse IL-10, recombinant rat IL-10, and recombinant cotton rat IL-10 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

1 mg

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.

Olson BM, Jankowska-Gan E, Becker JT, Vignali DA, Burlingham WJ, McNeel DG
Journal of immunology (Baltimore, Md. : 1950) 2012 Dec 15;189(12):5590-601

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Toll-like receptor 9 signaling by CpG-B oligodeoxynucleotides induces an apoptotic pathway in human chronic lymphocytic leukemia B cells.

Liang X, Moseman EA, Farrar MA, Bachanova V, Weisdorf DJ, Blazar BR, Chen W
Blood 2010 Jun 17;115(24):5041-52

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Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock?

Rojahn A, Brusletto B, Øvstebø R, Haug KB, Kierulf P, Brandtzaeg P
Critical care medicine 2008 Sep;36(9):2583-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human IL-10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-10 Polyclonal Antibody (Catalog # AB-217-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human IL-10 by Simple Western^TM. Simple Western lane view shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10, loaded at 0.2 mg/mL. A specific band was detected for IL-10 at approximately 23 kDa (as indicated) using 10 µg/mL of Goat Anti-Human IL-10 Polyclonal Antibody (Catalog # AB-217-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-10 and Neutralization by Human IL-10 Antibody. Recombinant Human IL-10 (Catalog # 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-10 Polyclonal Antibody (Catalog # AB-217-NA). The ND₅₀ is typically 3-15 µg/mL.