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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-417-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-10 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects mouse IL-10 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human IL-10 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation.
Low-dose lipopolysaccharide pretreatment suppresses choroidal neovascularization via IL-10 induction.
Histone deacetylase inhibitor LAQ824 augments inflammatory responses in macrophages through transcriptional regulation of IL-10.
Zhu L, Huang Z, Stålesen R, Hansson GK, Li N
Journal of thrombosis and haemostasis : JTH 2014 Jul;12(7):1156-65
Journal of thrombosis and haemostasis : JTH 2014 Jul;12(7):1156-65
Low-dose lipopolysaccharide pretreatment suppresses choroidal neovascularization via IL-10 induction.
Matsumura N, Kamei M, Tsujikawa M, Suzuki M, Xie P, Nishida K
PloS one 2012;7(7):e39890
PloS one 2012;7(7):e39890
Histone deacetylase inhibitor LAQ824 augments inflammatory responses in macrophages through transcriptional regulation of IL-10.
Wang H, Cheng F, Woan K, Sahakian E, Merino O, Rock-Klotz J, Vicente-Suarez I, Pinilla-Ibarz J, Wright KL, Seto E, Bhalla K, Villagra A, Sotomayor EM
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):3986-96
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):3986-96
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-10 and Neutralization by Mouse IL-10 Antibody. Recombinant Mouse IL-10 (Catalog # 417-ML) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-10 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-10 Polyclonal Antibody (Catalog # AB-417-NA). The ND50 is typically 2-4 µg/mL.
