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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- AF519 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-10 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse, rat, and cotton rat IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human IL-10, recombinant guinea pig IL-10, recombinant viral IL-10 and recombinant canine IL-10 is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Involvement of Interleukin-10 in Analgesia of Electroacupuncture on Incision Pain.
Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury.
Activation of GPR40 produces mechanical antiallodynia via the spinal glial interleukin-10/β-endorphin pathway.
Blocking p38 Signaling Reduces the Activation of Pro-inflammatory Cytokines and the Phosphorylation of p38 in the Habenula and Reverses Depressive-Like Behaviors Induced by Neuroinflammation.
Autocrine Interleukin-10 Mediates Glucagon-Like Peptide-1 Receptor-Induced Spinal Microglial β-Endorphin Expression.
ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury.
Y RNA fragment in extracellular vesicles confers cardioprotection via modulation of IL-10 expression and secretion.
A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFNγ.
Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during Colonization.
Cerebral regulatory T cells restrain microglia/macrophage-mediated inflammatory responses via IL-10.
Immunization with a Myelin-Derived Antigen Activates the Brain's Choroid Plexus for Recruitment of Immunoregulatory Cells to the CNS and Attenuates Disease Progression in a Mouse Model of ALS.
CNS repair requires both effector and regulatory T cells with distinct temporal and spatial profiles.
Serum-starved adipose-derived stromal cells ameliorate crescentic GN by promoting immunoregulatory macrophages.
Functionalized scaffold-mediated interleukin 10 gene delivery significantly improves survival rates of stem cells in vivo.
Ocular regulatory T cells distinguish monophasic from recurrent autoimmune uveitis.
Dai WJ, Sun JL, Li C, Mao W, Huang YK, Zhao ZQ, Zhang YQ, Lü N
Evidence-based complementary and alternative medicine : eCAM 2019;2019:8413576
Evidence-based complementary and alternative medicine : eCAM 2019;2019:8413576
Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury.
Zilberman-Itskovich S, Abu-Hamad R, Zarura R, Sova M, Hachmo Y, Stark M, Neuman S, Slavin S, Efrati S
PloS one 2019;14(9):e0222354
PloS one 2019;14(9):e0222354
Activation of GPR40 produces mechanical antiallodynia via the spinal glial interleukin-10/β-endorphin pathway.
Mao XF, Wu HY, Tang XQ, Ali U, Liu H, Wang YX
Journal of neuroinflammation 2019 Apr 13;16(1):84
Journal of neuroinflammation 2019 Apr 13;16(1):84
Blocking p38 Signaling Reduces the Activation of Pro-inflammatory Cytokines and the Phosphorylation of p38 in the Habenula and Reverses Depressive-Like Behaviors Induced by Neuroinflammation.
Zhao YW, Pan YQ, Tang MM, Lin WJ
Frontiers in pharmacology 2018;9:511
Frontiers in pharmacology 2018;9:511
Autocrine Interleukin-10 Mediates Glucagon-Like Peptide-1 Receptor-Induced Spinal Microglial β-Endorphin Expression.
Wu HY, Tang XQ, Mao XF, Wang YX
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Nov 29;37(48):11701-11714
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Nov 29;37(48):11701-11714
ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury.
Yang Y, Liu H, Zhang H, Ye Q, Wang J, Yang B, Mao L, Zhu W, Leak RK, Xiao B, Lu B, Chen J, Hu X
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 May 3;37(18):4692-4704
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 May 3;37(18):4692-4704
Y RNA fragment in extracellular vesicles confers cardioprotection via modulation of IL-10 expression and secretion.
Cambier L, de Couto G, Ibrahim A, Echavez AK, Valle J, Liu W, Kreke M, Smith RR, Marbán L, Marbán E
EMBO molecular medicine 2017 Mar;9(3):337-352
EMBO molecular medicine 2017 Mar;9(3):337-352
A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFNγ.
Lim JE, Chung E, Son Y
Scientific reports 2017 Aug 25;7(1):9417
Scientific reports 2017 Aug 25;7(1):9417
Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during Colonization.
Viladomiu M, Bassaganya-Riera J, Tubau-Juni N, Kronsteiner B, Leber A, Philipson CW, Zoccoli-Rodriguez V, Hontecillas R
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 15;198(8):3195-3204
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 15;198(8):3195-3204
Cerebral regulatory T cells restrain microglia/macrophage-mediated inflammatory responses via IL-10.
Xie L, Choudhury GR, Winters A, Yang SH, Jin K
European journal of immunology 2015 Jan;45(1):180-91
European journal of immunology 2015 Jan;45(1):180-91
Immunization with a Myelin-Derived Antigen Activates the Brain's Choroid Plexus for Recruitment of Immunoregulatory Cells to the CNS and Attenuates Disease Progression in a Mouse Model of ALS.
Kunis G, Baruch K, Miller O, Schwartz M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Apr 22;35(16):6381-93
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Apr 22;35(16):6381-93
CNS repair requires both effector and regulatory T cells with distinct temporal and spatial profiles.
Raposo C, Graubardt N, Cohen M, Eitan C, London A, Berkutzki T, Schwartz M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Jul 30;34(31):10141-55
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Jul 30;34(31):10141-55
Serum-starved adipose-derived stromal cells ameliorate crescentic GN by promoting immunoregulatory macrophages.
Furuhashi K, Tsuboi N, Shimizu A, Katsuno T, Kim H, Saka Y, Ozaki T, Sado Y, Imai E, Matsuo S, Maruyama S
Journal of the American Society of Nephrology : JASN 2013 Mar;24(4):587-603
Journal of the American Society of Nephrology : JASN 2013 Mar;24(4):587-603
Functionalized scaffold-mediated interleukin 10 gene delivery significantly improves survival rates of stem cells in vivo.
Holladay C, Power K, Sefton M, O'Brien T, Gallagher WM, Pandit A
Molecular therapy : the journal of the American Society of Gene Therapy 2011 May;19(5):969-78
Molecular therapy : the journal of the American Society of Gene Therapy 2011 May;19(5):969-78
Ocular regulatory T cells distinguish monophasic from recurrent autoimmune uveitis.
Ke Y, Jiang G, Sun D, Kaplan HJ, Shao H
Investigative ophthalmology & visual science 2008 Sep;49(9):3999-4007
Investigative ophthalmology & visual science 2008 Sep;49(9):3999-4007
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Recombinant Mouse and Rat IL-10 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL-10 (Catalog # 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-10 and Neutralization by Rat IL-10 Antibody. Recombinant Rat IL-10 (Catalog # 522-RL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519). The ND50 is typically 0.3-1.0 µg/mL.
