Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF-417-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-10 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant rat IL-10 and recombinant cotton rat IL-10 is observed, and less than 5% cross-reactivity with recombinant porcine IL-10, recombinant canine IL-10, recombinant feline IL-10, recombinant equine IL-10, recombinant viral IL-10, recombinant guinea pig IL-10, and recombinant human IL-10 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
Mutual modulation between interleukin-10 and interleukin-6 induced by Rhodococcus aurantiacus infection in mice.
Vascular and inflammatory stresses mediate atherosclerosis via RAGE and its ligands in apoE-/- mice.
El-Behi M, Ciric B, Dai H, Yan Y, Cullimore M, Safavi F, Zhang GX, Dittel BN, Rostami A
Nature immunology 2011 Jun;12(6):568-75
Nature immunology 2011 Jun;12(6):568-75
Mutual modulation between interleukin-10 and interleukin-6 induced by Rhodococcus aurantiacus infection in mice.
Yimin, Kohanawa M, Ozaki M, Haga S, Fujikawa K, Zhao S, Kuge Y, Tamaki N
Microbes and infection 2008 Nov-Dec;10(14-15):1450-8
Microbes and infection 2008 Nov-Dec;10(14-15):1450-8
Vascular and inflammatory stresses mediate atherosclerosis via RAGE and its ligands in apoE-/- mice.
Harja E, Bu DX, Hudson BI, Chang JS, Shen X, Hallam K, Kalea AZ, Lu Y, Rosario RH, Oruganti S, Nikolla Z, Belov D, Lalla E, Ramasamy R, Yan SF, Schmidt AM
The Journal of clinical investigation 2008 Jan;118(1):183-94
The Journal of clinical investigation 2008 Jan;118(1):183-94
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Recombinant Mouse IL-10 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL-10 (Catalog # 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-10 and Neutralization by Mouse IL-10 Antibody. Recombinant Mouse IL-10 (Catalog # 417-ML) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-10 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-417-NA). The ND50 is typically 0.01-0.05 µg/mL.