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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
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- Product number
- GTX15625 - Provider product page
- Provider
- GeneTex
- Product name
- IL10 antibody [9D7]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Simian
- Host
- Rat
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sandwich ELISA analysis of human IL10 protein in triplicate at 600, 240, 96, 38.4, 15.36, and 0 pg/ml (50 ul per wel) using GTX15625 as capture antibody (2 ug/ml) and GTX15764 as detection antibody (0.3 ug/ml). The plate was washed and incubated with Streptavidin-HRP. Detection was performed using TMB substrate. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
