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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
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- Product number
- MAB6931 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects porcine IL-10 in direct ELISAs. In direct ELISAs, 25-50% cross-reactivity with recombinant feline IL-10, recombinant canine IL-10, and recombinant guinea pig IL-10 is observed, approximately 10% cross-reactivity with recombinant human IL-10 is observed, less than 5% cross-reactivity with recombinant cotton rat IL-10 is observed, and no cross-reactivity with recombinant equine IL-10, recombinant mouse IL-10, recombinant rat IL-10, or recombinant viral IL-10 is observed.
- Reactivity
- Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Differential Effects of Escherichia coli Nissle and Lactobacillus rhamnosus Strain GG on Human Rotavirus Binding, Infection, and B Cell Immunity.
Postnatal persistent infection with classical Swine Fever virus and its immunological implications.
The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia.
Kandasamy S, Vlasova AN, Fischer D, Kumar A, Chattha KS, Rauf A, Shao L, Langel SN, Rajashekara G, Saif LJ
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 15;196(4):1780-9
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 15;196(4):1780-9
Postnatal persistent infection with classical Swine Fever virus and its immunological implications.
Muñoz-González S, Ruggli N, Rosell R, Pérez LJ, Frías-Leuporeau MT, Fraile L, Montoya M, Cordoba L, Domingo M, Ehrensperger F, Summerfield A, Ganges L
PloS one 2015;10(5):e0125692
PloS one 2015;10(5):e0125692
The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia.
Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tønnesen E
Intensive care medicine 2007 Jun;33(6):1085-93
Intensive care medicine 2007 Jun;33(6):1085-93
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-10 and Neutralization by Porcine IL-10 Antibody. Recombinant Porcine IL-10 (Catalog # 693-PI) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-10 Mono-clonal Antibody (Catalog # MAB6931). The ND50 is typically 0.05-0.15 µg/mL.
