MAB736

antibody from Novus Biologicals

Targeting: IL10 CSIF, IL-10, IL10A, TGIF

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB736

Provider

Novus Biologicals

Product name

Mouse Monoclonal IL-10 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects feline IL-10 in direct ELISAs and Western blots. In direct ELISAs, 40% cross-reactivity with canine IL-10 is observed and no cross-reactivity with IL-10 from human, mouse, rat, Epstein-Barr virus, or human cytomegalovirus is observed.

Reactivity

Feline

Host

Mouse

Isotype

IgG

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-10 and Neutralization by Feline IL-10 Antibody. Recombinant Feline IL-10 (Catalog # 736-FL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Feline IL-10 Monoclonal Antibody (Catalog # MAB736). The ND₅₀ is typically 0.2-0.6 µg/mL.