Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Western blot [2]
- Blocking/Neutralizing [1]
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- Product number
- MAB217-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-10 in Western blots. In Western blots, this antibody shows approximately 5% cross-reactivity with recombinant mouse IL-10.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P22301
- Isotype
- IgG
- Antibody clone number
- 23738
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Idelalisib and caffeine reduce suppression of T cell responses mediated by activated chronic lymphocytic leukemia cells.
Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages.
Fusion of ubiquitin to HIV gag impairs human monocyte-derived dendritic cell maturation and reduces ability to induce gag T cell responses.
TNF-α blockade induces IL-10 expression in human CD4+ T cells.
Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts.
Differential signaling of cmvIL-10 through common variants of the IL-10 receptor 1.
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Hepatitis C virus (HCV)-specific CD8+ cells produce transforming growth factor beta that can suppress HCV-specific T-cell responses.
Induction of host chemotactic response by Encephalitozoon spp.
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Increased frequency of CD4+ cells expressing CD161 in cancer patients.
Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
Distinct transcriptional programs activated by interleukin-10 with or without lipopolysaccharide in dendritic cells: induction of the B cell-activating chemokine, CXC chemokine ligand 13.
In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
IL-15 and cognate antigen successfully expand de novo-induced human antigen-specific regulatory CD4+ T cells that require antigen-specific activation for suppression.
Regulatory activity of autocrine IL-10 on dendritic cell functions.
Hock BD, MacPherson SA, McKenzie JL
PloS one 2017;12(3):e0172858
PloS one 2017;12(3):e0172858
Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages.
Huynh L, Kusnadi A, Park SH, Murata K, Park-Min KH, Ivashkiv LB
Scientific reports 2016 Aug 25;6:31959
Scientific reports 2016 Aug 25;6:31959
Fusion of ubiquitin to HIV gag impairs human monocyte-derived dendritic cell maturation and reduces ability to induce gag T cell responses.
Herath S, Benlahrech A, Papagatsias T, Athanasopoulos T, Bouzeboudjen Z, Hervouet C, Klavinskis L, Meiser A, Kelleher P, Dickson G, Patterson S
PloS one 2014;9(2):e88327
PloS one 2014;9(2):e88327
TNF-α blockade induces IL-10 expression in human CD4+ T cells.
Evans HG, Roostalu U, Walter GJ, Gullick NJ, Frederiksen KS, Roberts CA, Sumner J, Baeten DL, Gerwien JG, Cope AP, Geissmann F, Kirkham BW, Taams LS
Nature communications 2014;5:3199
Nature communications 2014;5:3199
Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts.
Heine G, Drozdenko G, Grün JR, Chang HD, Radbruch A, Worm M
European journal of immunology 2014 Jun;44(6):1615-21
European journal of immunology 2014 Jun;44(6):1615-21
Differential signaling of cmvIL-10 through common variants of the IL-10 receptor 1.
Gruber SG, Gloria Luciani M, Grundtner P, Zdanov A, Gasche C
European journal of immunology 2008 Dec;38(12):3365-75
European journal of immunology 2008 Dec;38(12):3365-75
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood 2007 May 15;109(10):4272-9
Blood 2007 May 15;109(10):4272-9
Hepatitis C virus (HCV)-specific CD8+ cells produce transforming growth factor beta that can suppress HCV-specific T-cell responses.
Alatrakchi N, Graham CS, van der Vliet HJ, Sherman KE, Exley MA, Koziel MJ
Journal of virology 2007 Jun;81(11):5882-92
Journal of virology 2007 Jun;81(11):5882-92
Induction of host chemotactic response by Encephalitozoon spp.
Fischer J, West J, Agochukwu N, Suire C, Hale-Donze H
Infection and immunity 2007 Apr;75(4):1619-25
Infection and immunity 2007 Apr;75(4):1619-25
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Ebert EC, Mehta V
Cellular immunology 2006 May;241(1):7-13
Cellular immunology 2006 May;241(1):7-13
Increased frequency of CD4+ cells expressing CD161 in cancer patients.
Iliopoulou EG, Karamouzis MV, Missitzis I, Ardavanis A, Sotiriadou NN, Baxevanis CN, Rigatos G, Papamichail M, Perez SA
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Dec 1;12(23):6901-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Dec 1;12(23):6901-9
Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
Blood 2005 Jan 15;105(2):750-8
Blood 2005 Jan 15;105(2):750-8
Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
Phillips TM
Electrophoresis 2004 Jun;25(10-11):1652-9
Electrophoresis 2004 Jun;25(10-11):1652-9
Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
Immunology 2004 Jun;112(2):274-89
Immunology 2004 Jun;112(2):274-89
Distinct transcriptional programs activated by interleukin-10 with or without lipopolysaccharide in dendritic cells: induction of the B cell-activating chemokine, CXC chemokine ligand 13.
Perrier P, Martinez FO, Locati M, Bianchi G, Nebuloni M, Vago G, Bazzoni F, Sozzani S, Allavena P, Mantovani A
Journal of immunology (Baltimore, Md. : 1950) 2004 Jun 1;172(11):7031-42
Journal of immunology (Baltimore, Md. : 1950) 2004 Jun 1;172(11):7031-42
In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB
Blood 2004 Jul 15;104(2):453-61
Blood 2004 Jul 15;104(2):453-61
IL-15 and cognate antigen successfully expand de novo-induced human antigen-specific regulatory CD4+ T cells that require antigen-specific activation for suppression.
Koenen HJ, Fasse E, Joosten I
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 15;171(12):6431-41
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 15;171(12):6431-41
Regulatory activity of autocrine IL-10 on dendritic cell functions.
Corinti S, Albanesi C, la Sala A, Pastore S, Girolomoni G
Journal of immunology (Baltimore, Md. : 1950) 2001 Apr 1;166(7):4312-8
Journal of immunology (Baltimore, Md. : 1950) 2001 Apr 1;166(7):4312-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB217) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-10 by Simple WesternTM. Simple Western lane view shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10, loaded at 0.2 mg/mL. A specific band was detected for IL-10 at approximately 23 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB217) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-10 and Neutralization by Human IL-10 Antibody. Recombinant Human IL-10 (Catalog # 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB217). At 5 µg/mL, this antibody will neutralize >60% Recombinant Human IL-10 induced bioactivity.