Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB915 - Provider product page
- Provider
- R&D Systems
- Product name
- Viral EBV IL-10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects viral EBV IL-10 in direct ELISAs and Western blots. In Western blots, less than 10% cross-reactivity with recombinant canine IL-10, recombinant feline IL-10, recombinant mouse IL-10, recombinant porcine IL-10, and recombinant rat IL-10 is observed.
- Reactivity
- Viral
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P03180
- Isotype
- IgG
- Antibody clone number
- 134216
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct.
Förster S, Brandt M, Mottok DS, Zschüttig A, Zimmermann K, Blattner FR, Gunzer F, Pöhlmann C
BMC biotechnology 2013 Oct 5;13:82
BMC biotechnology 2013 Oct 5;13:82
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-10 and Neutralization by Viral IL-10 Antibody. Recombinant Viral EBV IL-10 (Catalog # 915-VL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Viral EBV IL-10 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Viral EBV IL-10 Monoclonal Antibody (Catalog # MAB915). The ND50 is typically 0.1-0.4 µg/mL.