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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB7352 - Provider product page
- Provider
- R&D Systems
- Product name
- Canine IL-10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects canine IL-10 in Western blots.
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
XP_855560- Isotype
- IgG
- Antibody clone number
- 138128
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Recovery of antigen-specific T cell responses from dogs infected with Leishmania (L.) infantum by use of vaccine associated TLR-agonist adjuvant.
Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.
Schaut RG, Grinnage-Pulley TL, Esch KJ, Toepp AJ, Duthie MS, Howard RF, Reed SG, Petersen CA
Vaccine 2016 Oct 17;34(44):5225-5234
Vaccine 2016 Oct 17;34(44):5225-5234
Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.
Figueiredo MM, Amorim IF, Pinto AJ, Barbosa VS, Pinheiro Lde J, Deoti B, Faria AM, Tafuri WL
BMC immunology 2013 May 14;14:22
BMC immunology 2013 May 14;14:22
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-10 and Neutralization by Canine IL-10 Antibody. Recombinant Canine IL-10 (Catalog # 735-CL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Canine IL-10 Monoclonal Antibody (Catalog # MAB7352). The ND50 is typically 0.1-0.25 µg/mL.
