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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB736 - Provider product page
- Provider
- R&D Systems
- Product name
- Feline IL-10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects feline IL-10 in direct ELISAs and Western blots. In direct ELISAs, 40% cross-reactivity with canine IL-10 is observed and no cross-reactivity with IL-10 from human, mouse, rat, Epstein-Barr virus, or human cytomegalovirus is observed.
- Reactivity
- Feline
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P55029- Isotype
- IgG
- Antibody clone number
- 137706
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-10 and Neutralization by Feline IL-10 Antibody. Recombinant Feline IL-10 (Catalog # 736-FL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Feline IL-10 Monoclonal Antibody (Catalog # MAB736). The ND50 is typically 0.2-0.6 µg/mL.
