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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- GTX130513 - Provider product page
- Provider
- GeneTex
- Product name
- IL10 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IL10 antibody detects IL10 protein by western blot analysis. Non-transfected (-) and IL10-transfected (+) 293T whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody (GTX130513) diluted at 1:5000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- An ELISA plate is coated with 50 £gL of a IL10 peptide at concentration ranged from 0.25 £gg/mL to 8 £gg/mL. The coated peptide is detected with anti IL10 antibody (GTX130513) at 0.5 £gg/mL.
