R30118

antibody from NSJ Bioreagents

Targeting: IL10 CSIF, IL-10, IL10A, TGIF

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R30118

Provider

NSJ Bioreagents

Product name

Rat Il10 Antibody

Antibody type

Polyclonal

Description

This highly specific Rat Il10 antibody is suitable for use in Western blot/Immunohistochemistry/ELISA applications with rat samples.

Reactivity

Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Il10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of IL10 antibody and Lane 1: Recombinant rat protein 10ng; 2: 5ng; 3: 2.5ng

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Il10 antibody at 0.5ug/ml and recombinant rat protein: 10ng/lane, 5ng/lane and 1ng/lane.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat spleen tissue with Il10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.