AP52181PU-N

antibody from OriGene

Targeting: IL10 CSIF, IL-10, IL10A, TGIF

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP52181PU-N

Provider

OriGene

Product name

IL10 (Center) rabbit polyclonal antibody, Aff - Purified

Antibody type

Polyclonal

Description

IL10 (Center) rabbit polyclonal antibody, Aff - Purified

Host

Rabbit

Conjugate

Unconjugated

Epitope

IL10

Antibody clone number

NULL

Vial size

400 µl

Concentration

0.5 mg/ml (Lot specific)

References

Submitted references

Neuroinflammatory signals in Alzheimer disease and APP/PS1 transgenic mice: correlations with plaques, tangles, and oligomeric species.

López-González I, Schlüter A, Aso E, Garcia-Esparcia P, Ansoleaga B, LLorens F, Carmona M, Moreno J, Fuso A, Portero-Otin M, Pamplona R, Pujol A, Ferrer I
Journal of neuropathology and experimental neurology 2015 Apr;74(4):319-44

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of IL10 (arrow) in MDA-MB435 cell line lysates (35ug/lane) using Interleukin-10 / IL10 antibody . .

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Human breast carcinoma (formalin-fixed, paraffin-embedded) reacted with IL10 antibody .?, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for IHC; clinical relevance has not been evaluated.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram) using Interleukin-10/IL10 antibody . . FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Validation comment

FC