H00003586-M03

antibody from Abnova Corporation

Targeting: IL10 CSIF, IL-10, IL10A, TGIF

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003586-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003586-M03, RRID:AB_894148

Product name

IL10 monoclonal antibody (M03), clone 1C10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant IL10.

Antigen sequence

FKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAH
VNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVK
NAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN


Isotype

IgG

Antibody clone number

1C10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of IL10 expression in transfected 293T cell line by IL10 monoclonal antibody (M03), clone 1C10.Lane 1: IL10 transfected lysate(20.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged IL10 is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol