Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB9210-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human IL-10 in direct ELISAs.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P22301
- Isotype
- IgG
- Antibody clone number
- 2050B
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-10 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) stimulated to induce Th2 cells or (B) unstimulated were stained with Rabbit Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB9210) followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112) and Mouse Anti-Human CD4 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB3791N). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-10 and Neutralization by Human IL-10 Antibody. Recombinant Human IL-10 (Catalog # 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB9210). The ND50 is typically 75-450 ng/mL.