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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Flow cytometry [1]
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- Product number
- 53-7108-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IL-10 Monoclonal Antibody (JES3-9D7), Alexa Fluor 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10). Applications Reported: This JES3-9D7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- JES3-9D7
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Multiple Sclerosis-Associated Changes in the Composition and Immune Functions of Spore-Forming Bacteria.
Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.
Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.
β-Catenin Stabilization in Skin Fibroblasts Causes Fibrotic Lesions by Preventing Adipocyte Differentiation of the Reticular Dermis.
The regulation of cancer cell migration by lung cancer cell-derived exosomes through TGF-β and IL-10.
Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.
CD4(+)CD25(-)Foxp3(+) T cells play a role in tuberculous hydrothorax rather than malignant hydrothorax.
Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.
Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.
Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.
Role of the phosphatidylinositol 3 kinase-Akt pathway in the regulation of IL-10 and IL-12 by Porphyromonas gingivalis lipopolysaccharide.
Cekanaviciute E, Pröbstel AK, Thomann A, Runia TF, Casaccia P, Katz Sand I, Crabtree E, Singh S, Morrissey J, Barba P, Gomez R, Knight R, Mazmanian S, Graves J, Cree BAC, Zamvil SS, Baranzini SE
mSystems 2018 Nov-Dec;3(6)
mSystems 2018 Nov-Dec;3(6)
Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.
Singh S, Vedi S, Samrat SK, Li W, Kumar R, Agrawal B
PloS one 2016;11(1):e0146404
PloS one 2016;11(1):e0146404
Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.
Mariuzzi L, Domenis R, Orsaria M, Marzinotto S, Londero AP, Bulfoni M, Candotti V, Zanello A, Ballico M, Mimmi MC, Calcagno A, Marchesoni D, Di Loreto C, Beltrami AP, Cesselli D, Gri G
Laboratory investigation; a journal of technical methods and pathology 2016 Sep;96(9):959-971
Laboratory investigation; a journal of technical methods and pathology 2016 Sep;96(9):959-971
β-Catenin Stabilization in Skin Fibroblasts Causes Fibrotic Lesions by Preventing Adipocyte Differentiation of the Reticular Dermis.
Mastrogiannaki M, Lichtenberger BM, Reimer A, Collins CA, Driskell RR, Watt FM
The Journal of investigative dermatology 2016 Jun;136(6):1130-1142
The Journal of investigative dermatology 2016 Jun;136(6):1130-1142
The regulation of cancer cell migration by lung cancer cell-derived exosomes through TGF-β and IL-10.
Wang Y, Yi J, Chen X, Zhang Y, Xu M, Yang Z
Oncology letters 2016 Feb;11(2):1527-1530
Oncology letters 2016 Feb;11(2):1527-1530
Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.
Miles B, Miller SM, Folkvord JM, Kimball A, Chamanian M, Meditz AL, Arends T, McCarter MD, Levy DN, Rakasz EG, Skinner PJ, Connick E
Nature communications 2015 Oct 20;6:8608
Nature communications 2015 Oct 20;6:8608
CD4(+)CD25(-)Foxp3(+) T cells play a role in tuberculous hydrothorax rather than malignant hydrothorax.
Tang Y, Peng LP, Qin GX, Sun JT, Xu LJ, Jiang YF
Journal of translational medicine 2015 Aug 18;13:268
Journal of translational medicine 2015 Aug 18;13:268
Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.
Nowacki TM, Kuerten S, Zhang W, Shive CL, Kreher CR, Boehm BO, Lehmann PV, Tary-Lehmann M
Cellular immunology 2007 May;247(1):36-48
Cellular immunology 2007 May;247(1):36-48
Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.
Sendide K, Deghmane AE, Pechkovsky D, Av-Gay Y, Talal A, Hmama Z
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):5324-32
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):5324-32
Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.
Sendide K, Deghmane AE, Pechkovsky D, Av-Gay Y, Talal A, Hmama Z
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):5324-32
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):5324-32
Role of the phosphatidylinositol 3 kinase-Akt pathway in the regulation of IL-10 and IL-12 by Porphyromonas gingivalis lipopolysaccharide.
Martin M, Schifferle RE, Cuesta N, Vogel SN, Katz J, Michalek SM
Journal of immunology (Baltimore, Md. : 1950) 2003 Jul 15;171(2):717-25
Journal of immunology (Baltimore, Md. : 1950) 2003 Jul 15;171(2):717-25
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of Th2-polarized cells restimulated with PMA/Ionomycin (right) or unstimulated (left) in the presence of monensin for 5 hrs. Cells were harvested, stained with Anti-Human CD3 PE (Product # 12-0037-42) and Anti-Human IL-10 Alexa Fluor® 488.
- Conjugate
- Green dye
