M00021-1

antibody from Boster Biological Technology

Targeting: IL10 CSIF, IL-10, IL10A, TGIF

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

M00021-1

Provider

Boster Biological Technology

Product name

Anti- IL-10 Monoclonal Antibody

Antibody type

Monoclonal

Description

Rat IgG monoclonal antibody for IL-10 detection. Tested with WB, IHC-P in Mouse.

Reactivity

Mouse

Host

Rat

Antibody clone number

AFIE-9

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

References

Submitted references

Pi-Pa-Run-Fei-Tang alleviates lung injury by modulating IL-6/JAK2/STAT3/IL-17 and PI3K/AKT/NF-κB signaling pathway and balancing Th17 and Treg in murine model of OVA-induced asthma.

Jie XL, Luo ZR, Yu J, Tong ZR, Li QQ, Wu JH, Tao Y, Feng PS, Lan JP, Wang P
Journal of ethnopharmacology 2023 Dec 5;317:116719

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Olfactory mucosa tissue-derived mesenchymal stem cells lysate ameliorates LPS-induced acute liver injury in mice.

Wang Z, Zhang X, Qi L, Feng W, Gu Y, Ding Y
BMC pulmonary medicine 2022 Nov 11;22(1):414

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Novel Multifunctional Silver Nanocomposite Serves as a Resistance-Reversal Agent to Synergistically Combat Carbapenem-Resistant Acinetobacter baumannii.

Li X, Gui R, Li J, Huang R, Shang Y, Zhao Q, Liu H, Jiang H, Shang X, Wu X, Nie X
ACS applied materials & interfaces 2021 Jul 7;13(26):30434-30457

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Bufei Yishen Granules Combined with Acupoint Sticking Therapy Suppress Inflammation in Chronic Obstructive Pulmonary Disease Rats: Via JNK/p38 Signaling Pathway.

Tian Y, Li Y, Li J, Feng S, Li S, Mao J, Xie Y, Liu X, Dong H, Zheng W, Wang M
Evidence-based complementary and alternative medicine : eCAM 2017;2017:1768243

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Inhibition of Tumor Growth and Immunomodulatory Effects of Flavonoids and Scutebarbatines of Scutellaria barbata D. Don in Lewis-Bearing C57BL/6 Mice.

Gong T, Wang CF, Yuan JR, Li Y, Gu JF, Zhao BJ, Zhang L, Jia XB, Feng L, Liu SL
Evidence-based complementary and alternative medicine : eCAM 2015;2015:630760

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of IL-10 using anti- IL-10 antibody (M00021-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse IL-10 protein 15ng, Lane 2: recombinant mouse IL-10 protein 10ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rat anti- IL-10 antigen affinity purified monoclonal antibody (Catalog # M00021-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.

Additional image