Ab109811

antibody from Aladdin Scientific

Targeting: IL10 CSIF, IL-10, IL10A, TGIF

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab109811

Provider

Aladdin Scientific

Product name

IL-10 Mouse mAb

Antibody type

Monoclonal

Description

Mouse anti Human IL-10 Antibody, Monoclonal (3G12-7), is a Capture antibody could be used for ELISA, CLIA, LF, GICA, FIA, FACS. CLIA: 20µg/2mg magnetic beadsFLFIA: Fluorescent beads/Antibody = 10/1

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

3G12-7

Vial size

100_g,10_g,1mg,50_g

Concentration

Lot by Lot

Storage

Store at 37¡C short term_7 days_Store at 4¡C short term_30 days_. Store at -20±5¡C long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

ELISA

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Sandwich ELISA - IL-10 protein standard curve Human IL-10 protein was serially diluted 2-fold and captured by IL-10 Mouse mAb (Ab109811) coated on a Clear Polystyrene Microplate (W291037). IL-10 Mouse mAb (Ab109817) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Binding ELISA - IL-10 Mouse mAb (Ab109811) Immobilized Recombinant Human IL-10 Protein at 1.0 μg/mL can bind IL-10 Mouse mAb (Ab109811) with the EC₅₀ of 31.66 ng/mL.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Relevance of detection results between aladdin Antibody Pair (Ab109811 & Ab109817) in CLIA ·Capture antibody: IL-10 Mouse mAb (Ab109811) ·Detection antibody: IL-10 Mouse mAb (Ab109817)