Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- ELISA [3]
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Validation data
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- Product number
- Ab109811 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- IL-10 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human IL-10 Antibody, Monoclonal (3G12-7), is a Capture antibody could be used for ELISA, CLIA, LF, GICA, FIA, FACS. CLIA: 20µg/2mg magnetic beadsFLFIA: Fluorescent beads/Antibody = 10/1
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 3G12-7
- Vial size
- 100_g,10_g,1mg,50_g
- Concentration
- Lot by Lot
- Storage
- Store at 37¡C short term_7 days_Store at 4¡C short term_30 days_. Store at -20±5¡C long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Sandwich ELISA - IL-10 protein standard curve Human IL-10 protein was serially diluted 2-fold and captured by IL-10 Mouse mAb (Ab109811) coated on a Clear Polystyrene Microplate (W291037). IL-10 Mouse mAb (Ab109817) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Binding ELISA - IL-10 Mouse mAb (Ab109811) Immobilized Recombinant Human IL-10 Protein at 1.0 μg/mL can bind IL-10 Mouse mAb (Ab109811) with the EC₅₀ of 31.66 ng/mL.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Relevance of detection results between aladdin Antibody Pair (Ab109811 & Ab109817) in CLIA ·Capture antibody: IL-10 Mouse mAb (Ab109811) ·Detection antibody: IL-10 Mouse mAb (Ab109817)