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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP52181PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52181PU-N, RRID:AB_11144726
- Product name
- anti Interleukin-10 / IL10 (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the center region (between 27-53aa) of human IL10.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of IL10 (arrow) in MDA-MB435 cell line lysates (35ug/lane) using Interleukin-10 / IL10 antibody Cat.-No. AP52181PU-N.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human breast carcinoma (formalin-fixed, paraffin-embedded) reacted with IL10 antibody Cat.-No. AP52181PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for IHC; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram) using Interleukin-10/IL10 antibody Cat.-No. AP52181PU-N. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
