Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AB-217-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-10 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant equine IL-10, recombinant canine IL-10, recombinant porcine IL-10, and recombinant feline IL-10 is observed, and less than 10% cross-reactivity with recombinant mouse IL-10, recombinant rat IL-10, and recombinant cotton rat IL-10 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P22301
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
Toll-like receptor 9 signaling by CpG-B oligodeoxynucleotides induces an apoptotic pathway in human chronic lymphocytic leukemia B cells.
Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock?
Olson BM, Jankowska-Gan E, Becker JT, Vignali DA, Burlingham WJ, McNeel DG
Journal of immunology (Baltimore, Md. : 1950) 2012 Dec 15;189(12):5590-601
Journal of immunology (Baltimore, Md. : 1950) 2012 Dec 15;189(12):5590-601
Toll-like receptor 9 signaling by CpG-B oligodeoxynucleotides induces an apoptotic pathway in human chronic lymphocytic leukemia B cells.
Liang X, Moseman EA, Farrar MA, Bachanova V, Weisdorf DJ, Blazar BR, Chen W
Blood 2010 Jun 17;115(24):5041-52
Blood 2010 Jun 17;115(24):5041-52
Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock?
Rojahn A, Brusletto B, Øvstebø R, Haug KB, Kierulf P, Brandtzaeg P
Critical care medicine 2008 Sep;36(9):2583-9
Critical care medicine 2008 Sep;36(9):2583-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-10 Polyclonal Antibody (Catalog # AB-217-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-10 by Simple WesternTM. Simple Western lane view shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10, loaded at 0.2 mg/mL. A specific band was detected for IL-10 at approximately 23 kDa (as indicated) using 10 µg/mL of Goat Anti-Human IL-10 Polyclonal Antibody (Catalog # AB-217-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-10 and Neutralization by Human IL-10 Antibody. Recombinant Human IL-10 (Catalog # 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-10 Polyclonal Antibody (Catalog # AB-217-NA). The ND50 is typically 3-15 µg/mL.