Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
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Validation data
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- Product number
- LS-B3923 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ GRB2 Antibody (aa206-217) LS-B3923
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Hamster, Horse, Porcine, Rabbit, Simian
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Staining (2 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat Anti-GRB2 Antibody (0.03µg/ml) staining of Human (A) and Pig (B) Spleen lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat Anti-GRB2 Antibody (0.05µg/ml) staining of Human Thymus (A) and MOLT4 (B) lysates (35µg total protein in RIPA buffer). Primary incubated for 1 hour. Detected by chemiluminescencence.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Goat Anti-GRB2 Antibody (0.05µg/ml) staining of Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates (35µg total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.