PA1-31495

antibody from Invitrogen Antibodies

Targeting: GRB2 NCKAP2

Antibody data

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Validation data

Product number: PA1-31495 - Provider product page
Provider: Invitrogen Antibodies
Product name: GRB2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX22234-PEP).. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat, Porcine
Host: Goat
Isotype: IgG
Vial size: 50 µg
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

GRB2 protein structure - PA1-31495 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GRB2 using a GRB2 polyclonal antibody (Product # PA1-31495).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GRB2 in Jurkat lysate (RIPA buffer, 35g total protein per lane) using a GRB2 polyclonal antibody (Product # PA1-31495) at a dilution of 2g/mL following incubation for 1 hour and detected using ECL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of GRB2 was performed by loading 35 µg (in RIPA buffer) of human (A) and pig (B) spleen lysates. Proteins were transferred to a membrane and probed with a GRB2 Polyclonal Antibody (Product # PA1-31495) at a dilution of 0.03 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of GRB2 was performed by loading 35 µg (in RIPA buffer) of human (A) and pig (B) spleen lysates. Proteins were transferred to a membrane and probed with a GRB2 Polyclonal Antibody (Product # PA1-31495) at a dilution of 0.03 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of GRB2 was performed by loading 35 µg (in RIPA buffer) of human (A) and pig (B) spleen lysates. Proteins were transferred to a membrane and probed with a GRB2 Polyclonal Antibody (Product # PA1-31495) at a dilution of 0.03 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of GRB2 was performed by loading 35 µg (in RIPA buffer) of human thymus (A) and Molt-4 (B) lysates. Proteins were transferred to a membrane and probed with a GRB2 Polyclonal Antibody (Product # PA1-31495) at a dilution of 0.05 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of GRB2 was performed by loading 35 µg (in RIPA buffer) of mouse (A+C) and rat (B+D) brain (A+B) and spleen (C+D) lysates. Proteins were transferred to a membrane and probed with a GRB2 Polyclonal Antibody (Product # PA1-31495) at a dilution of 0.05 µg/mL.