10254-2-AP

antibody from Proteintech Group

Targeting: GRB2 NCKAP2

Provider product page for 10254-2-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [24]
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Validations
Western blot [1]

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Product number: 10254-2-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10254-2-AP, RRID:AB_2263577
Product name: GRB2 antibody
Antibody type: Polyclonal
Description: KD/KO validated GRB2 antibody (Cat. #10254-2-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

GRB2 protein structure - 10254-2-AP shown in red.

Submitted references Exploring the Anti-Cervical Cancer Effect and Hepatotoxicity Risk of Gossypol Based on Untargeted Metabolomics and Network Toxicology.

Li J, Parhat P, Ma Y, Peng L, Li M
Pharmaceuticals (Basel, Switzerland) 2026 Feb 27;19(3)

Augmented recruitment of host Grb2 by Helicobacter pylori tyrosine phosphorylated CagA EPIYA-D motif promotes cellular oncogenic transformation.

Zhao H, Liu S, Shan J, Zhang J, Wu Q, Zhang J, Sun Z, Cao X, Chen S, Feng M, Zhang Y, Cheng X, Li B, Ji X
Biochimica et biophysica acta. Molecular basis of disease 2025 Nov;1871(8):168018

GRB2 Promotes Malignant Behaviors of Breast Cancer by Modulating the Global Expression and Alternative Splicing Profiles in SK-BR-3 Cells Through Binding mRNA.

Liu W, Huang Y, Qiao L, Chong L, Xia L, Abudurehaman A, Li H
Cancer medicine 2025 May;14(10):e70905

ROR1 facilitates glioblastoma growth via stabilizing GRB2 to promote c-Fos expression in glioma stem cells.

Zhu H, Cheng L, Liu D, Ma X, Chen Z, Fan H, Li R, Zhang Y, Mi H, Li J, Zhang S, Yu X, Shu K
Neuro-oncology 2025 Mar 7;27(3):695-710

Molecular Target Identification of Gossypol Against Cervical Cancer Based on Target Fishing Technology.

Li J, Asat R, Li W, Parhat P, Ma Y, Ma Y, Li M
Pharmaceutics 2025 Jun 30;17(7)

Osteoblast-Derived ECM1 Promotes Anti-Androgen Resistance in Bone Metastatic Prostate Cancer.

Wang X, Wang M, Lin Q, He L, Zhang B, Chen X, Chen G, Du H, Lang C, Peng X, Dai Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jan;12(2):e2407662

Targeting neuregulin-1 in cartilage homeostasis restoration: Kaempferol-3-o-rutinoside as a novel treatment for osteoarthritis.

Sheng Y, Li S, Wang X, Cui P, Wang Y, Wang Y, Zhang Y, Wang Q, Wang C, Tao J, Wu C, Jiang X
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Dec;149:157562

DDX42 Enhances Hepatocellular Carcinoma Cell Proliferation, Radiation and Sorafenib Resistance via Regulating GRB2 RNA Maturation and Activating PI3K/AKT Pathway.

Liu Z, Yuan J, Liu F, Zeng Q, Wu Z, Yang J
Journal of cellular and molecular medicine 2025 Aug;29(16):e70793

Bufalin inhibits hepatocellular carcinoma progression by blocking EGFR-mediated RAS-RAF-MEK-ERK pathway activation.

Liu J, Jiang J, Huang J, Fang ZE, Liu L, Liu Y, Nian W, Tang J, Wang Z
Journal of experimental & clinical cancer research : CR 2025 Aug 29;44(1):260

TGF-β-induced lncRNA TBUR1 promotes EMT and metastasis in lung adenocarcinoma via hnRNPC-mediated GRB2 mRNA stabilization.

Huang L, Liu X, Chen Q, Yang J, Zhang D, Zhao Y, Xu L, Li Z, Liu X, Shao S, Li D, Song Y, Liu X, Zhan Q
Cancer letters 2024 Sep 28;600:217153

Extraction, purification and anticancer activity studies on triterpenes from pomegranate peel.

Zhang M, Zhang Y, Guo X, Chen Y, Li H, Zhou G, Sun S, Ren Q, Simal-Gandara J, Sun J, Li N, Liu C
Food & function 2024 Jul 1;15(13):6914-6928

Screening and Verification of Key Ubiquitination Genes Related to Immune Infiltration in Stage III/IV Hepatocellular Carcinoma.

Tang Y, Cao J, Peng R, Mao X, Su B, Tang H, Tu D, Zhou J, Jiang G, Jin S, Wang Q, Zhang C, Liu R, Zhang C, Bai D
Journal of hepatocellular carcinoma 2023;10:765-781

Glucose-mediated N-glycosylation of RPTPα affects its subcellular localization and Src activation.

Fang J, Zhang Y, Huang C, Lu R, Yu J, Chen R, Wang Y, Zhao X, Yu J, Huang J
Oncogene 2023 Mar;42(14):1058-1071

In vivo CRISPR screens identify RhoV as a pro-metastasis factor of triple-negative breast cancer.

Jin ML, Gong Y, Ji P, Hu X, Shao ZM
Cancer science 2023 Jun;114(6):2375-2385

HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis.

Jiang Q, Zhang D, Liu J, Liang C, Yang R, Zhang C, Wu J, Lin J, Ye T, Ding L, Li J, Gao S, Li B, Ye Q
Science advances 2023 Jun 9;9(23):eade1155

Elevated PGT promotes proliferation and inhibits cell apoptosis in preeclampsia by Erk signaling pathway.

Pang H, Lei D, Huang J, Guo Y, Fan C
Molecular and cellular probes 2023 Feb;67:101896

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Chu H, Zhao Q, Liu J, Yang K, Wang Y, Liu J, Zhang K, Zhao B, He H, Zheng Y, Zhong S, Liang Z, Zhang L, Zhang Y
Analytical chemistry 2022 Jan 18;94(2):758-767

Deubiquitinase PSMD14 enhances hepatocellular carcinoma growth and metastasis by stabilizing GRB2.

Lv J, Zhang S, Wu H, Lu J, Lu Y, Wang F, Zhao W, Zhan P, Lu J, Fang Q, Xie C, Yin Z
Cancer letters 2020 Jan 28;469:22-34

ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway.

Meng L, Liu S, Liu F, Sang M, Ju Y, Fan X, Gu L, Li Z, Geng C, Sang M
Molecular therapy. Nucleic acids 2020 Aug 19;22:124-137

MicroRNA-133b/EGFR axis regulates esophageal squamous cell carcinoma metastases by suppressing anoikis resistance and anchorage-independent growth.

Zhu JF, Liu Y, Huang H, Shan L, Han ZG, Liu JY, Li YL, Dong X, Zeng W
Cancer cell international 2018;18:193

Spiroplasma eriocheiris induces mouse 3T6-Swiss albino cell apoptosis that associated with the infection mechanism.

Hou L, Gu W, Zhu H, Yao W, Wang W, Meng Q
Molecular immunology 2017 Nov;91:75-85

Melittin inhibits tumor growth and decreases resistance to gemcitabine by downregulating cholesterol pathway gene CLU in pancreatic ductal adenocarcinoma.

Wang X, Xie J, Lu X, Li H, Wen C, Huo Z, Xie J, Shi M, Tang X, Chen H, Peng C, Fang Y, Deng X, Shen B
Cancer letters 2017 Jul 28;399:1-9

iTRAQ-based Proteomic Analysis of Porcine Kidney Epithelial PK15 cells Infected with Pseudorabies virus.

Yang S, Pei Y, Zhao A
Scientific reports 2017 Apr 4;7:45922

mir-329 restricts tumor growth by targeting grb2 in pancreatic cancer.

Wang X, Lu X, Zhang T, Wen C, Shi M, Tang X, Chen H, Peng C, Li H, Fang Y, Deng X, Shen B
Oncotarget 2016 Apr 19;7(16):21441-53

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The GRB2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human GRB2. This antibody recognizes human,mouse,rat antigen. The GRB2 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 10254-2-AP.pdf

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