STJ70104

antibody from St John's Laboratory

Targeting: GRB2 NCKAP2

Western blot

ELISA

Antibody data

Submit

Validation data

Product number: STJ70104 - Provider product page
Provider: St John's Laboratory
Product name: Anti-GRB2 antibody (C-Term) (STJ70104)
Antibody type: Polyclonal
Description: Goat polyclonal antibody anti-GRB2 (C-Term) is suitable for use in ELISA and Western Blot research applications.
Reactivity: Human, Mouse, Rat, Bovine, Canine
Host: Goat
Conjugate: Unconjugated
Antigen sequence: PRNYVTPVNRNV
Epitope: NA
Isotype: IgG
Antibody clone number: NA
Vial size: NA
Concentration: NA
Storage: Store at-20 on receipt and minimise freeze-thaw cycles.
Handling: NA

GRB2 protein structure - STJ70104 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: STJ70104 (0. 05µg/ml) staining of Human Thymus (A) and MOLT4 (B) lysates (35µg total protein in RIPA buffer). Primary incubated for 1 hour. Detected by chemiluminescence.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: STJ70104 (0. 03µg/ml) staining of Human (A) and Pig (B) Spleen lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: STJ70104 (0. 05µg/ml) staining of Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates (35µg total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: STJ70104 (1µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: STJ70104 Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA