MA5-35238

antibody from Invitrogen Antibodies

Targeting: GRB2 NCKAP2

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-35238

Provider

Invitrogen Antibodies

Product name

GRB2 Recombinant Rabbit Monoclonal Antibody (9K6H0)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: YFLWVVKFNS LNELVDYHRS TSVSRNQQIF LRDIEQVPQQ PTYVQALFDF DPQEDGELGF RRGDFIHVMD NSDPNWWKGA CHGQTGMFPR NYVTPVNRNV

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

9K6H0

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of GRB2 performed on HeLa cells using a GRB2 monoclonal antibody (Product # MA5-35238) at a dilution of 1:100. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of GRB2 performed on HeLa cells using a GRB2 monoclonal antibody (Product # MA5-35238) at a dilution of 1:100. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal imaging (Immunocytochemistry) of GRB2 in HeLa cells. Samples were incubated with GRB2 Monoclonal antibody (Product # MA5-35238) using a dilution of 1:100 (Red). The cells were counterstained with alpha Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of GRB2 in 300 μg extracts from 293T cells. Samples were precipitated with 3 μg GRB2 Monoclonal antibody (Product # MA5-35238). Western blot was performed from the immunoprecipitate using GRB2 Monoclonal antibody (Product # MA5-35238) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GRB2 in paraffin-embedded human lung cancer. Samples were incubated with GRB2 Monoclonal antibody (Product # MA5-35238) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GRB2 in paraffin-embedded rat spleen. Samples were incubated with GRB2 Monoclonal antibody (Product # MA5-35238) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GRB2 in paraffin-embedded mouse spleen. Samples were incubated with GRB2 Monoclonal antibody (Product # MA5-35238) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.