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- References [6]
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- Product number
- ABIN1449302 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (N-Term) antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant Ash/Grb-2
- Description
- Ammonium sulfate precipitation and affinity chromatography on protein A
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Epitope
- N-Term
- Isotype
- IgG
- Antibody clone number
- 3F2
- Vial size
- 0.1 mg
- Storage
- Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted (in aliquots) at -20°C.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Grb2 mediates the EGF-dependent activation of guanine nucleotide exchange on Ras.
Guanine-nucleotide-releasing factor hSos1 binds to Grb2 and links receptor tyrosine kinases to Ras signalling.
The SH2 and SH3 domains of mammalian Grb2 couple the EGF receptor to the Ras activator mSos1.
Cloning of ASH, a ubiquitous protein composed of one Src homology region (SH) 2 and two SH3 domains, from human and rat cDNA libraries.
C. elegans cell-signalling gene sem-5 encodes a protein with SH2 and SH3 domains.
The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.
Gale NW, Kaplan S, Lowenstein EJ, Schlessinger J, Bar-Sagi D
Nature 1993 May 6;363(6424):88-92
Nature 1993 May 6;363(6424):88-92
Guanine-nucleotide-releasing factor hSos1 binds to Grb2 and links receptor tyrosine kinases to Ras signalling.
Li N, Batzer A, Daly R, Yajnik V, Skolnik E, Chardin P, Bar-Sagi D, Margolis B, Schlessinger J
Nature 1993 May 6;363(6424):85-8
Nature 1993 May 6;363(6424):85-8
The SH2 and SH3 domains of mammalian Grb2 couple the EGF receptor to the Ras activator mSos1.
Rozakis-Adcock M, Fernley R, Wade J, Pawson T, Bowtell D
Nature 1993 May 6;363(6424):83-5
Nature 1993 May 6;363(6424):83-5
Cloning of ASH, a ubiquitous protein composed of one Src homology region (SH) 2 and two SH3 domains, from human and rat cDNA libraries.
Matuoka K, Shibata M, Yamakawa A, Takenawa T
Proceedings of the National Academy of Sciences of the United States of America 1992 Oct 1;89(19):9015-9
Proceedings of the National Academy of Sciences of the United States of America 1992 Oct 1;89(19):9015-9
C. elegans cell-signalling gene sem-5 encodes a protein with SH2 and SH3 domains.
Clark SG, Stern MJ, Horvitz HR
Nature 1992 Mar 26;356(6367):340-4
Nature 1992 Mar 26;356(6367):340-4
The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.
Lowenstein EJ, Daly RJ, Batzer AG, Li W, Margolis B, Lammers R, Ullrich A, Skolnik EY, Bar-Sagi D, Schlessinger J
Cell 1992 Aug 7;70(3):431-42
Cell 1992 Aug 7;70(3):431-42
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- Image(s): Western Blotting
