Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AP3254a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP3254a, RRID:AB_2196167
- Product name
- Bi-Phospho-YES1(Y537)/Phospho-SRC(Y530) Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Phosphorylation of collapsin response mediator protein 2 on Tyr-479 regulates CXCL12-induced T lymphocyte migration.
Varrin-Doyer M, Vincent P, Cavagna S, Auvergnon N, Noraz N, Rogemond V, Honnorat J, Moradi-Améli M, Giraudon P
The Journal of biological chemistry 2009 May 8;284(19):13265-76
The Journal of biological chemistry 2009 May 8;284(19):13265-76
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Dot blot analysis of anti-Phospho-YES1-Y537/Phospho-SRC-Y530 Antibody (Cat.#AP3254a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
- Primary Ab dilution
- 1:500