Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- 44-655G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SRC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Dysregulated PDGFRα signaling alters coronal suture morphogenesis and leads to craniosynostosis through endochondral ossification.
Tumor endothelial cells promote metastasis and cancer stem cell-like phenotype through elevated Epiregulin in esophageal cancer.
MEK1 binds directly to betaarrestin1, influencing both its phosphorylation by ERK and the timing of its isoprenaline-stimulated internalization.
Analyzing FAK and Pyk2 in early integrin signaling events.
He F, Soriano P
Development (Cambridge, England) 2017 Nov 1;144(21):4026-4036
Development (Cambridge, England) 2017 Nov 1;144(21):4026-4036
Tumor endothelial cells promote metastasis and cancer stem cell-like phenotype through elevated Epiregulin in esophageal cancer.
Sun L, Pan J, Yu L, Liu H, Shu X, Sun L, Lou J, Yang Z, Ran Y
American journal of cancer research 2016;6(10):2277-2288
American journal of cancer research 2016;6(10):2277-2288
MEK1 binds directly to betaarrestin1, influencing both its phosphorylation by ERK and the timing of its isoprenaline-stimulated internalization.
Meng D, Lynch MJ, Huston E, Beyermann M, Eichhorst J, Adams DR, Klussmann E, Houslay MD, Baillie GS
The Journal of biological chemistry 2009 Apr 24;284(17):11425-35
The Journal of biological chemistry 2009 Apr 24;284(17):11425-35
Analyzing FAK and Pyk2 in early integrin signaling events.
Bernard-Trifilo JA, Lim ST, Hou S, Schlaepfer DD, Ilic D
Current protocols in cell biology 2006 Apr;Chapter 14:Unit 14.7
Current protocols in cell biology 2006 Apr;Chapter 14:Unit 14.7
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot. Extracts prepared from CEF cells transfected with Src (1) or left untransfected (2) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for two hours at room temperature and incubated with a 1:1000 dilution of Src pan antibody for two hours at room temperature in a 1% BSA-TBST buffer. After washing, membranes were incubated with goat F (ab’)2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and bands were detected using the Pierce SuperSignal™ method.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot. Extracts prepared from CEF cells transfected with Src (1) or left untransfected (2) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for two hours at room temperature and incubated with a 1:1000 dilution of Src pan antibody for two hours at room temperature in a 1% BSA-TBST buffer. After washing, membranes were incubated with goat F (ab’)2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and bands were detected using the Pierce SuperSignal™ method.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SRC showing staining in the cytoplasm of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a SRC polyclonal antibody (Product # 44-655G) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL