- 44-662G - Invitrogen Antibodies SRC antibody

Submitted references Acetone Extract of Cornus officinalis Leaves Exerts Anti-Melanoma Effects via Inhibiting STAT3 Signaling.

Xu R, Zeng M, Wu Y, Wang S, Zhang B, Zhang J, Kan Y, Li B, Cao B, Zheng X, Feng W
OncoTargets and therapy 2021;14:3487-3501

LY6D-induced macropinocytosis as a survival mechanism of senescent cells.

Nagano T, Iwasaki T, Onishi K, Awai Y, Terachi A, Kuwaba S, Asano S, Katasho R, Nagai K, Nakashima A, Kikkawa U, Kamada S
The Journal of biological chemistry 2021 Jan-Jun;296:100049

CDCP1 promotes compensatory renal growth by integrating Src and Met signaling.

Kajiwara K, Yamano S, Aoki K, Okuzaki D, Matsumoto K, Okada M
Life science alliance 2021 Apr;4(4)

Fyn is an intermediate kinase that BDNF utilizes to promote oligodendrocyte myelination.

Peckham H, Giuffrida L, Wood R, Gonsalvez D, Ferner A, Kilpatrick TJ, Murray SS, Xiao J
Glia 2016 Feb;64(2):255-69

Hyaluronan oligosaccharides promote diabetic wound healing by increasing angiogenesis.

Wang Y, Han G, Guo B, Huang J
Pharmacological reports : PR 2016 Dec;68(6):1126-1132

Phosphatidylinositol phosphate 5-kinase Iγi2 in association with Src controls anchorage-independent growth of tumor cells.

Thapa N, Choi S, Hedman A, Tan X, Anderson RA
The Journal of biological chemistry 2013 Nov 29;288(48):34707-18

An osteoclastic protein-tyrosine phosphatase regulates the β3-integrin, syk, and shp1 signaling through respective src-dependent phosphorylation in osteoclasts.

Lau KH, Stiffel V, Amoui M
American journal of physiology. Cell physiology 2012 Jun 1;302(11):C1676-86

Acquisition of a potent and selective TC-PTP inhibitor via a stepwise fluorophore-tagged combinatorial synthesis and screening strategy.

Zhang S, Chen L, Luo Y, Gunawan A, Lawrence DS, Zhang ZY
Journal of the American Chemical Society 2009 Sep 16;131(36):13072-9

Analyzing FAK and Pyk2 in early integrin signaling events.

Bernard-Trifilo JA, Lim ST, Hou S, Schlaepfer DD, Ilic D
Current protocols in cell biology 2006 Apr;Chapter 14:Unit 14.7

A mechanosensory complex that mediates the endothelial cell response to fluid shear stress.

Tzima E, Irani-Tehrani M, Kiosses WB, Dejana E, Schultz DA, Engelhardt B, Cao G, DeLisser H, Schwartz MA
Nature 2005 Sep 15;437(7057):426-31

Reelin activates SRC family tyrosine kinases in neurons.

Bock HH, Herz J
Current biology : CB 2003 Jan 8;13(1):18-26

Reelin activates SRC family tyrosine kinases in neurons.

Bock HH, Herz J
Current biology : CB 2003 Jan 8;13(1):18-26

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using SRC (pY529) Polyclonal Antibody, Rabbit

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 synchronized overnight (Lane 1), A549 synchronized and treated with 50 ng/mL PDGF for 10 minutes (Lane 2), SH-SY5Y synchronized overnight (Lane 3), SH-SY5Y synchronized overnight and treated with 50 ng/mL PDGF for 10 minutes (Lane 4), HT-29 synchronized overnight (Lane 5), HT-29 synchronized overnight and treated with 200 ng/mL EGF for 10 minutes (Lane 6).The blots were probed with SRC (pY529) Rabbit Polyclonal Antibody (Product # 44-662G, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 55 kDa band corresponding to Phospho-SRC pTyr529 was enhanced across cell lines treated either with PDGF or EGF. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of SRC (pY529) was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with SRC (pY529) Rabbit Polyclonal Antibody (Product # 44-662G) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing Nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of SRC showing staining in the cytoplasm of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a SRC polyclonal antibody (Product # 44-662G) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of SRC showing staining in the cytoplasm and membrane of paraffin-embedded mouse colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a SRC polyclonal antibody (Product # 44-662G) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of SRC [pY529] was done on A549 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with SRC [pY529] Rabbit Polyclonal Antibody (44662G, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

44-662G

Antibody data