Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 44-662G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-SRC (Tyr529) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Acetone Extract of Cornus officinalis Leaves Exerts Anti-Melanoma Effects via Inhibiting STAT3 Signaling.
LY6D-induced macropinocytosis as a survival mechanism of senescent cells.
CDCP1 promotes compensatory renal growth by integrating Src and Met signaling.
Fyn is an intermediate kinase that BDNF utilizes to promote oligodendrocyte myelination.
Hyaluronan oligosaccharides promote diabetic wound healing by increasing angiogenesis.
Phosphatidylinositol phosphate 5-kinase Iγi2 in association with Src controls anchorage-independent growth of tumor cells.
An osteoclastic protein-tyrosine phosphatase regulates the β3-integrin, syk, and shp1 signaling through respective src-dependent phosphorylation in osteoclasts.
Acquisition of a potent and selective TC-PTP inhibitor via a stepwise fluorophore-tagged combinatorial synthesis and screening strategy.
Analyzing FAK and Pyk2 in early integrin signaling events.
A mechanosensory complex that mediates the endothelial cell response to fluid shear stress.
Reelin activates SRC family tyrosine kinases in neurons.
Reelin activates SRC family tyrosine kinases in neurons.
Xu R, Zeng M, Wu Y, Wang S, Zhang B, Zhang J, Kan Y, Li B, Cao B, Zheng X, Feng W
OncoTargets and therapy 2021;14:3487-3501
OncoTargets and therapy 2021;14:3487-3501
LY6D-induced macropinocytosis as a survival mechanism of senescent cells.
Nagano T, Iwasaki T, Onishi K, Awai Y, Terachi A, Kuwaba S, Asano S, Katasho R, Nagai K, Nakashima A, Kikkawa U, Kamada S
The Journal of biological chemistry 2021 Jan-Jun;296:100049
The Journal of biological chemistry 2021 Jan-Jun;296:100049
CDCP1 promotes compensatory renal growth by integrating Src and Met signaling.
Kajiwara K, Yamano S, Aoki K, Okuzaki D, Matsumoto K, Okada M
Life science alliance 2021 Apr;4(4)
Life science alliance 2021 Apr;4(4)
Fyn is an intermediate kinase that BDNF utilizes to promote oligodendrocyte myelination.
Peckham H, Giuffrida L, Wood R, Gonsalvez D, Ferner A, Kilpatrick TJ, Murray SS, Xiao J
Glia 2016 Feb;64(2):255-69
Glia 2016 Feb;64(2):255-69
Hyaluronan oligosaccharides promote diabetic wound healing by increasing angiogenesis.
Wang Y, Han G, Guo B, Huang J
Pharmacological reports : PR 2016 Dec;68(6):1126-1132
Pharmacological reports : PR 2016 Dec;68(6):1126-1132
Phosphatidylinositol phosphate 5-kinase Iγi2 in association with Src controls anchorage-independent growth of tumor cells.
Thapa N, Choi S, Hedman A, Tan X, Anderson RA
The Journal of biological chemistry 2013 Nov 29;288(48):34707-18
The Journal of biological chemistry 2013 Nov 29;288(48):34707-18
An osteoclastic protein-tyrosine phosphatase regulates the β3-integrin, syk, and shp1 signaling through respective src-dependent phosphorylation in osteoclasts.
Lau KH, Stiffel V, Amoui M
American journal of physiology. Cell physiology 2012 Jun 1;302(11):C1676-86
American journal of physiology. Cell physiology 2012 Jun 1;302(11):C1676-86
Acquisition of a potent and selective TC-PTP inhibitor via a stepwise fluorophore-tagged combinatorial synthesis and screening strategy.
Zhang S, Chen L, Luo Y, Gunawan A, Lawrence DS, Zhang ZY
Journal of the American Chemical Society 2009 Sep 16;131(36):13072-9
Journal of the American Chemical Society 2009 Sep 16;131(36):13072-9
Analyzing FAK and Pyk2 in early integrin signaling events.
Bernard-Trifilo JA, Lim ST, Hou S, Schlaepfer DD, Ilic D
Current protocols in cell biology 2006 Apr;Chapter 14:Unit 14.7
Current protocols in cell biology 2006 Apr;Chapter 14:Unit 14.7
A mechanosensory complex that mediates the endothelial cell response to fluid shear stress.
Tzima E, Irani-Tehrani M, Kiosses WB, Dejana E, Schultz DA, Engelhardt B, Cao G, DeLisser H, Schwartz MA
Nature 2005 Sep 15;437(7057):426-31
Nature 2005 Sep 15;437(7057):426-31
Reelin activates SRC family tyrosine kinases in neurons.
Bock HH, Herz J
Current biology : CB 2003 Jan 8;13(1):18-26
Current biology : CB 2003 Jan 8;13(1):18-26
Reelin activates SRC family tyrosine kinases in neurons.
Bock HH, Herz J
Current biology : CB 2003 Jan 8;13(1):18-26
Current biology : CB 2003 Jan 8;13(1):18-26
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SRC (pY529) Polyclonal Antibody, Rabbit
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 synchronized overnight (Lane 1), A549 synchronized and treated with 50 ng/mL PDGF for 10 minutes (Lane 2), SH-SY5Y synchronized overnight (Lane 3), SH-SY5Y synchronized overnight and treated with 50 ng/mL PDGF for 10 minutes (Lane 4), HT-29 synchronized overnight (Lane 5), HT-29 synchronized overnight and treated with 200 ng/mL EGF for 10 minutes (Lane 6).The blots were probed with SRC (pY529) Rabbit Polyclonal Antibody (Product # 44-662G, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 55 kDa band corresponding to Phospho-SRC pTyr529 was enhanced across cell lines treated either with PDGF or EGF. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SRC (pY529) was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with SRC (pY529) Rabbit Polyclonal Antibody (Product # 44-662G) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing Nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SRC showing staining in the cytoplasm of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a SRC polyclonal antibody (Product # 44-662G) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SRC showing staining in the cytoplasm and membrane of paraffin-embedded mouse colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a SRC polyclonal antibody (Product # 44-662G) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of SRC [pY529] was done on A549 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with SRC [pY529] Rabbit Polyclonal Antibody (44662G, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL