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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX32265 - Provider product page
- Provider
- GeneTex
- Product name
- Src (phospho Tyr527) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of c-SRC (pY529) expression in K562 (A); HuvEc (B); Jurkat (C); COLO205 (D); HEK293T EGF-treated (E); A431 pervanadate-treated (F) whole cell lysates.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of c-SRC (pY529) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
