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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
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- Product number
- MAB1155 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1155, RRID:AB_1582164
- Product name
- SRC monoclonal antibody, clone 17AT28
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant SRC.
- Isotype
- IgG
- Antibody clone number
- 17AT28
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Expression of a Src family kinase in chronic myelogenous leukemia cells induces resistance to imatinib in a kinase-dependent manner.
Human phosphatidylethanolamine-binding protein 4 promotes transactivation of estrogen receptor alpha (ERalpha) in human cancer cells by inhibiting proteasome-dependent ERalpha degradation via association with Src.
Microexon-based regulation of ITSN1 and Src SH3 domains specificity relies on introduction of charged amino acids into the interaction interface.
Pene-Dumitrescu T, Smithgall TE
The Journal of biological chemistry 2010 Jul 9;285(28):21446-57
The Journal of biological chemistry 2010 Jul 9;285(28):21446-57
Human phosphatidylethanolamine-binding protein 4 promotes transactivation of estrogen receptor alpha (ERalpha) in human cancer cells by inhibiting proteasome-dependent ERalpha degradation via association with Src.
Liu H, Qiu J, Li N, Chen T, Cao X
The Journal of biological chemistry 2010 Jul 16;285(29):21934-42
The Journal of biological chemistry 2010 Jul 16;285(29):21934-42
Microexon-based regulation of ITSN1 and Src SH3 domains specificity relies on introduction of charged amino acids into the interaction interface.
Dergai M, Tsyba L, Dergai O, Zlatskii I, Skrypkina I, Kovalenko V, Rynditch A
Biochemical and biophysical research communications 2010 Aug 20;399(2):307-12
Biochemical and biophysical research communications 2010 Aug 20;399(2):307-12
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The SRC monoclonal antibody, clone 17AT28 (Cat # MAB1155) is used in Western blot to detect SRC in Jurkat cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the beta3 integrin subunit (FG-beta3) or a beta3 truncation mutant (FG-759x) . SRC monoclonal antibody, clone 17AT28 (Cat # MAB1155) was diluted in 1% BSA/TBST and incubated overnight at 4 °C. After washing 3 x 5 min with TBST the blots were incubated with goat anti-mouse or goat anti-rabbit secondary antibody for 1 hr at room temperature. The blots were again washed 3 x 5 min with TBST and developed using ECL reagent. Data is kindly provided by Dr. Weis of Cheresh Lab, UCSD.
