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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [14]
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- Validations
- Flow cytometry [5]
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- Product number
- NB100-56560 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56560, RRID:AB_839181
- Product name
- Rat Monoclonal TLR4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. In FA, the antibody blocks activation of monocytes with LPS (Akashi et al. 2000). This antibody preferentially recognizes TLR4-MD-2 complex than of TLR4 alone.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The immunologically active oligosaccharides isolated from wheatgrass modulate monocytes via Toll-like receptor-2 signaling.
hsp70 and a novel axis of type I interferon-dependent antiviral immunity in the measles virus-infected brain.
Monocytic thrombomodulin triggers LPS- and gram-negative bacteria-induced inflammatory response.
Abnormal trafficking and degradation of TLR4 underlie the elevated inflammatory response in cystic fibrosis.
TLR4-mediated activation of mouse macrophages by Korean mistletoe lectin-C (KML-C).
Lipopolysaccharide-binding protein-mediated Toll-like receptor 4 dimerization enables rapid signal transduction against lipopolysaccharide stimulation on membrane-associated CD14-expressing cells.
Central role of MyD88-dependent dendritic cell maturation and proinflammatory cytokine production to control Brucella abortus infection.
Central role of MyD88-dependent dendritic cell maturation and proinflammatory cytokine production to control Brucella abortus infection.
TLR4 on follicular dendritic cells: an activation pathway that promotes accessory activity.
TLR4 on follicular dendritic cells: an activation pathway that promotes accessory activity.
Immune response to Candida albicans is preserved despite defect in O-mannosylation of secretory proteins.
A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression.
TLR4, but not TLR2, signals autoregulatory apoptosis of cultured microglia: a critical role of IFN-beta as a decision maker.
The matrix component biglycan is proinflammatory and signals through Toll-like receptors 4 and 2 in macrophages.
Tsai CC, Lin CR, Tsai HY, Chen CJ, Li WT, Yu HM, Ke YY, Hsieh WY, Chang CY, Wu YT, Chen ST, Wong CH
The Journal of biological chemistry 2013 Jun 14;288(24):17689-97
The Journal of biological chemistry 2013 Jun 14;288(24):17689-97
hsp70 and a novel axis of type I interferon-dependent antiviral immunity in the measles virus-infected brain.
Kim MY, Shu Y, Carsillo T, Zhang J, Yu L, Peterson C, Longhi S, Girod S, Niewiesk S, Oglesbee M
Journal of virology 2013 Jan;87(2):998-1009
Journal of virology 2013 Jan;87(2):998-1009
Monocytic thrombomodulin triggers LPS- and gram-negative bacteria-induced inflammatory response.
Ma CY, Shi GY, Shi CS, Kao YC, Lin SW, Wu HL
Journal of immunology (Baltimore, Md. : 1950) 2012 Jun 15;188(12):6328-37
Journal of immunology (Baltimore, Md. : 1950) 2012 Jun 15;188(12):6328-37
Abnormal trafficking and degradation of TLR4 underlie the elevated inflammatory response in cystic fibrosis.
Bruscia EM, Zhang PX, Satoh A, Caputo C, Medzhitov R, Shenoy A, Egan ME, Krause DS
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):6990-8
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):6990-8
TLR4-mediated activation of mouse macrophages by Korean mistletoe lectin-C (KML-C).
Park HJ, Hong JH, Kwon HJ, Kim Y, Lee KH, Kim JB, Song SK
Biochemical and biophysical research communications 2010 Jun 4;396(3):721-5
Biochemical and biophysical research communications 2010 Jun 4;396(3):721-5
Lipopolysaccharide-binding protein-mediated Toll-like receptor 4 dimerization enables rapid signal transduction against lipopolysaccharide stimulation on membrane-associated CD14-expressing cells.
Tsukamoto H, Fukudome K, Takao S, Tsuneyoshi N, Kimoto M
International immunology 2010 Apr;22(4):271-80
International immunology 2010 Apr;22(4):271-80
Central role of MyD88-dependent dendritic cell maturation and proinflammatory cytokine production to control Brucella abortus infection.
Macedo GC, Magnani DM, Carvalho NB, Bruna-Romero O, Gazzinelli RT, Oliveira SC
Journal of immunology (Baltimore, Md. : 1950) 2008 Jan 15;180(2):1080-7
Journal of immunology (Baltimore, Md. : 1950) 2008 Jan 15;180(2):1080-7
Central role of MyD88-dependent dendritic cell maturation and proinflammatory cytokine production to control Brucella abortus infection.
Macedo GC, Magnani DM, Carvalho NB, Bruna-Romero O, Gazzinelli RT, Oliveira SC
Journal of immunology (Baltimore, Md. : 1950) 2008 Jan 15;180(2):1080-7
Journal of immunology (Baltimore, Md. : 1950) 2008 Jan 15;180(2):1080-7
TLR4 on follicular dendritic cells: an activation pathway that promotes accessory activity.
El Shikh ME, El Sayed RM, Wu Y, Szakal AK, Tew JG
Journal of immunology (Baltimore, Md. : 1950) 2007 Oct 1;179(7):4444-50
Journal of immunology (Baltimore, Md. : 1950) 2007 Oct 1;179(7):4444-50
TLR4 on follicular dendritic cells: an activation pathway that promotes accessory activity.
El Shikh ME, El Sayed RM, Wu Y, Szakal AK, Tew JG
Journal of immunology (Baltimore, Md. : 1950) 2007 Oct 1;179(7):4444-50
Journal of immunology (Baltimore, Md. : 1950) 2007 Oct 1;179(7):4444-50
Immune response to Candida albicans is preserved despite defect in O-mannosylation of secretory proteins.
Corbucci C, Cenci E, Skrzypek F, Gabrielli E, Mosci P, Ernst JF, Bistoni F, Vecchiarelli A
Medical mycology 2007 Dec;45(8):709-19
Medical mycology 2007 Dec;45(8):709-19
A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression.
Konno K, Wakabayashi Y, Akashi-Takamura S, Ishii T, Kobayashi M, Takahashi K, Kusumoto Y, Saitoh S, Yoshizawa Y, Miyake K
Biochemical and biophysical research communications 2006 Jan 27;339(4):1076-82
Biochemical and biophysical research communications 2006 Jan 27;339(4):1076-82
TLR4, but not TLR2, signals autoregulatory apoptosis of cultured microglia: a critical role of IFN-beta as a decision maker.
Jung DY, Lee H, Jung BY, Ock J, Lee MS, Lee WH, Suk K
Journal of immunology (Baltimore, Md. : 1950) 2005 May 15;174(10):6467-76
Journal of immunology (Baltimore, Md. : 1950) 2005 May 15;174(10):6467-76
The matrix component biglycan is proinflammatory and signals through Toll-like receptors 4 and 2 in macrophages.
Schaefer L, Babelova A, Kiss E, Hausser HJ, Baliova M, Krzyzankova M, Marsche G, Young MF, Mihalik D, Götte M, Malle E, Schaefer RM, Gröne HJ
The Journal of clinical investigation 2005 Aug;115(8):2223-33
The Journal of clinical investigation 2005 Aug;115(8):2223-33
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide/BSA FREE version of NBP2-24865. Cell surface analysis of TLR4 on mouse RAW cells using 0.5 ug/10^6 cells of TLR4 anitbody, anti-rat APC conjugated secondary antibody and cell surface flow kit. Green: isotype control. Red: TLR4 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide Free version of NB100-56560. Cell surface TLR4 FITC in RAW cells using 0.5 ug of this antibody. Black: cells without antibody. Green: isotype control. Red: anti-TLR4 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using Azide Free version of NB100-56560. Cell surface TLR4 in mouse peritoneal cells. Green: isotype control. Red: anti-TLR4 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the PE conjugate of NBP2-24865. Staining of cell surface TLR4 using this antibody at 0.625 ug/10^6 Raw cells (pretreated with anti-CD16/32). Shaded histogram: isotype control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (MTS510) [NB100-56560] - Analysis using the FITC conjugate of NBP2-24865. Staining of TLR4 in mouse peritoneal cells using 1 ug of this antibody. Shaded histogram: Cells without antibody; green represents isotype control; red represents anti-TLR4 antibody.
