NBP2-24538

antibody from Novus Biologicals

Targeting: TLR4 ARMD10, CD284, hToll, TLR-4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-24538

Provider

Novus Biologicals

Product name

Rabbit Polyclonal TLR4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Bovine, Simian

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Myeloid-Derived Lymphatic Endothelial Cell Progenitors Significantly Contribute to Lymphatic Metastasis in Clinical Breast Cancer.

Volk-Draper L, Patel R, Bhattarai N, Yang J, Wilber A, DeNardo D, Ran S
The American journal of pathology 2019 Nov;189(11):2269-2292

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Ontogeny of white matter, toll-like receptor expression, and motor skills in the neonatal ferret.

Snyder JM, Wood TR, Corry K, Moralejo DH, Parikh P, Juul SE
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 2018 Nov;70:25-33

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IFN-γ alters the expression of diverse immunity related genes in a cell culture model designed to represent maturing neutrophils.

Ellison MA, Gearheart CM, Porter CC, Ambruso DR
PloS one 2017;12(10):e0185956

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Arginine Relieves the Inflammatory Response and Enhances the Casein Expression in Bovine Mammary Epithelial Cells Induced by Lipopolysaccharide.

Wu T, Wang C, Ding L, Shen Y, Cui H, Wang M, Wang H
Mediators of inflammation 2016;2016:9618795

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Activation of TLR4 is required for the synergistic induction of dual oxidase 2 and dual oxidase A2 by IFN-γ and lipopolysaccharide in human pancreatic cancer cell lines.

Wu Y, Lu J, Antony S, Juhasz A, Liu H, Jiang G, Meitzler JL, Hollingshead M, Haines DC, Butcher D, Roy K, Doroshow JH
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 15;190(4):1859-72

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Toll-like receptor 4 facilitates binding of Shiga toxin to colon carcinoma and primary umbilical vein endothelial cells.

Torgersen ML, Engedal N, Pedersen AM, Husebye H, Espevik T, Sandvig K
FEMS immunology and medical microbiology 2011 Feb;61(1):63-75

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TLR4-mediated activation of mouse macrophages by Korean mistletoe lectin-C (KML-C).

Park HJ, Hong JH, Kwon HJ, Kim Y, Lee KH, Kim JB, Song SK
Biochemical and biophysical research communications 2010 Jun 4;396(3):721-5

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TLR4 Antibody [NBP2-24538] - Analysis of TLR4 in human intestine lysate in the 1) absence and 2) presence of immunizing peptide using TLR4 antibody at 0.1 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TLR4 Antibody [NBP2-24538] - Lanes containing cell lysate from untreated cell are on the left (-) and lysates from IFNG treated cells are on the right (+). The proteins are identified by the corresponding gene names. The housekeeping enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was blotted as a control that is not altered by IFNG treatment. Except for GAPDH, the numbers under each gene name are the fold changes in mRNAs. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0185956) licensed under a CC-BY licence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TLR4 Antibody [NBP2-24538] - Human prostate stained with TLR4 antibody at 10 ug/ml.