NB100-56580-0.1mg

antibody from Novus Biologicals

Targeting: TLR4 ARMD10, CD284, hToll, TLR-4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-56580-0.1mg

Provider

Novus Biologicals

Product name

Rabbit Polyclonal TLR4 Antibody

Antibody type

Polyclonal

Description

Protein G purified. This antibody was tested against partial recombinant mouse TLR4 (extracellular portion), and a 75-80 kDa band was observed. Full-length TLR4 is observed at approximately 90 kD. 2. Zager et al (2006, Fig. 10) observed TLR4 as an ~95 kDa doublet which is thought to represent different degrees of glycosylation. See Product Citation (Renal Physiol 291:F546-F556) for additional information. 3. Presumptive TLR4 30 and 60 kDa cleavage fragments are described in Zagar et al 2006.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TLR4 Antibody [NB100-56580] - Analysis of TLR4 using this antibody. 1 ug/lane partial recombinant mouse TLR4 protein (tested at 2 ug/ml) and B) RAW cell lysate and C) Daudi cell lysate (tested at 5 ug/ml on tumor cell lysate).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TLR4 Antibody [NB100-56580] - Immunoblot data examining response of primary macrophages from WT and miR-223 knockout mice (KO) to LPS and combined IFNG/LPS; 50ug of whole cell lysate was loaded per lane and run on a denaturing SDS PAGE gel. Densitometry and statistics are presented in (D). All values are expressed as +/- SEM; * indicates p

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TLR4 Antibody [NB100-56580] - Analysis of TLR4 in mouse kidney tissue using TLR4 antibody at 5 ug/ml.