Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB2759P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal TLR4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse TLR4 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant mouse TLR1, 2, 3, 6, or recombinant human TLR4.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Morphine inhibits murine dendritic cell IL-23 production by modulating Toll-like receptor 2 and Nod2 signaling.
Wang J, Ma J, Charboneau R, Barke R, Roy S
The Journal of biological chemistry 2011 Mar 25;286(12):10225-32
The Journal of biological chemistry 2011 Mar 25;286(12):10225-32
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TLR4 in RAW 264.7 Mouse Cell Line by Flow Cytometry. LPS-activated RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TLR4 PE-conjugated Monoclonal Antibody (Catalog # FAB2759P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.