Antibody data
- Antibody Data
- Antigen structure
- References [27]
- Comments [0]
- Validations
- Flow cytometry [9]
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- Product number
- NB100-56727 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56727, RRID:AB_839179
- Product name
- Mouse Monoclonal TLR4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. The has been shown to block the activation of monocytes with LPS (Paik, et al, 2003).
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Nfa34810 Facilitates Nocardia farcinica Invasion of Host Cells and Stimulates Tumor Necrosis Factor Alpha Secretion through Activation of the NF-κB and Mitogen-Activated Protein Kinase Pathways via Toll-Like Receptor 4.
Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve Cells.
ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells.
Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.
Leishmania mexicana lipophosphoglycan activates ERK and p38 MAP kinase and induces production of proinflammatory cytokines in human macrophages through TLR2 and TLR4.
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
TAK-242 (resatorvid), a small-molecule inhibitor of Toll-like receptor (TLR) 4 signaling, binds selectively to TLR4 and interferes with interactions between TLR4 and its adaptor molecules.
TLR4-dependent induction of vascular adhesion molecule-1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A(2)alpha/cyclooxygenase-2.
Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia.
Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes.
HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum.
Stimulation of iNOS expression and apoptosis resistance in B-cell chronic lymphocytic leukemia (B-CLL) cells through engagement of Toll-like receptor 7 (TLR-7) and NF-kappaB activation.
Human lymphatic endothelial cells express multiple functional TLRs.
Human lymphatic endothelial cells express multiple functional TLRs.
Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid.
In vitro response to Candida albicans in cultures of whole human blood from young and aged donors.
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Execution of macrophage apoptosis by PE_PGRS33 of Mycobacterium tuberculosis is mediated by Toll-like receptor 2-dependent release of tumor necrosis factor-alpha.
RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes.
RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes.
Cell activation by ligands of the toll-like receptor and interleukin-1 receptor family depends on the function of the large-conductance potassium channel MaxiK in human macrophages.
A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression.
NF-kappaB- and C/EBPbeta-driven interleukin-1beta gene expression and PAK1-mediated caspase-1 activation play essential roles in interleukin-1beta release from Helicobacter pylori lipopolysaccharide-stimulated macrophages.
Evidence of Toll-like receptor molecules on human platelets.
Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2.
Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent.
Ji X, Zhang X, Li H, Sun L, Hou X, Song H, Han L, Xu S, Qiu X, Wang X, Zheng N, Li Z
Infection and immunity 2020 Mar 23;88(4)
Infection and immunity 2020 Mar 23;88(4)
Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve Cells.
The E, Yao Q, Zhang P, Zhai Y, Ao L, Fullerton DA, Meng X
Cells 2020 Feb 7;9(2)
Cells 2020 Feb 7;9(2)
ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells.
Li F, Song R, Ao L, Reece TB, Cleveland JC Jr, Dong N, Fullerton DA, Meng X
Arteriosclerosis, thrombosis, and vascular biology 2017 Jul;37(7):1339-1351
Arteriosclerosis, thrombosis, and vascular biology 2017 Jul;37(7):1339-1351
Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.
Käbisch R, Mejías-Luque R, Gerhard M, Prinz C
PloS one 2014;9(8):e104804
PloS one 2014;9(8):e104804
Leishmania mexicana lipophosphoglycan activates ERK and p38 MAP kinase and induces production of proinflammatory cytokines in human macrophages through TLR2 and TLR4.
Rojas-Bernabé A, Garcia-Hernández O, Maldonado-Bernal C, Delegado-Dominguez J, Ortega E, Gutiérrez-Kobeh L, Becker I, Aguirre-Garcia M
Parasitology 2014 May;141(6):788-800
Parasitology 2014 May;141(6):788-800
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
Harman AN, Bye CR, Nasr N, Sandgren KJ, Kim M, Mercier SK, Botting RA, Lewin SR, Cunningham AL, Cameron PU
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
TAK-242 (resatorvid), a small-molecule inhibitor of Toll-like receptor (TLR) 4 signaling, binds selectively to TLR4 and interferes with interactions between TLR4 and its adaptor molecules.
Matsunaga N, Tsuchimori N, Matsumoto T, Ii M
Molecular pharmacology 2011 Jan;79(1):34-41
Molecular pharmacology 2011 Jan;79(1):34-41
TLR4-dependent induction of vascular adhesion molecule-1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A(2)alpha/cyclooxygenase-2.
Wu CY, Chi PL, Hsieh HL, Luo SF, Yang CM
Journal of cellular physiology 2010 May;223(2):480-91
Journal of cellular physiology 2010 May;223(2):480-91
Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia.
Pillay J, Ramakers BP, Kamp VM, Loi AL, Lam SW, Hietbrink F, Leenen LP, Tool AT, Pickkers P, Koenderman L
Journal of leukocyte biology 2010 Jul;88(1):211-20
Journal of leukocyte biology 2010 Jul;88(1):211-20
Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes.
Pietschmann K, Beetz S, Welte S, Martens I, Gruen J, Oberg HH, Wesch D, Kabelitz D
Scandinavian journal of immunology 2009 Sep;70(3):245-55
Scandinavian journal of immunology 2009 Sep;70(3):245-55
HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum.
O'Hara SP, Small AJ, Gajdos GB, Badley AD, Chen XM, Larusso NF
The Journal of infectious diseases 2009 Apr 15;199(8):1195-204
The Journal of infectious diseases 2009 Apr 15;199(8):1195-204
Stimulation of iNOS expression and apoptosis resistance in B-cell chronic lymphocytic leukemia (B-CLL) cells through engagement of Toll-like receptor 7 (TLR-7) and NF-kappaB activation.
Hammadi A, Billard C, Faussat AM, Kolb JP
Nitric oxide : biology and chemistry 2008 Sep;19(2):138-45
Nitric oxide : biology and chemistry 2008 Sep;19(2):138-45
Human lymphatic endothelial cells express multiple functional TLRs.
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Human lymphatic endothelial cells express multiple functional TLRs.
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
Burgener IA, Jungi TW
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91
TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid.
Prabha C, Rajashree P, Sulochana DD
Immunology letters 2008 Apr 15;117(1):26-34
Immunology letters 2008 Apr 15;117(1):26-34
In vitro response to Candida albicans in cultures of whole human blood from young and aged donors.
Murciano C, Villamón E, Yáñez A, Murciano J, Mir A, O'Connor JE, Gozalbo D, Gil ML
FEMS immunology and medical microbiology 2007 Nov;51(2):327-35
FEMS immunology and medical microbiology 2007 Nov;51(2):327-35
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
Execution of macrophage apoptosis by PE_PGRS33 of Mycobacterium tuberculosis is mediated by Toll-like receptor 2-dependent release of tumor necrosis factor-alpha.
Basu S, Pathak SK, Banerjee A, Pathak S, Bhattacharyya A, Yang Z, Talarico S, Kundu M, Basu J
The Journal of biological chemistry 2007 Jan 12;282(2):1039-50
The Journal of biological chemistry 2007 Jan 12;282(2):1039-50
RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes.
Shahrara S, Park CC, Temkin V, Jarvis JW, Volin MV, Pope RM
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5077-87
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5077-87
RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes.
Shahrara S, Park CC, Temkin V, Jarvis JW, Volin MV, Pope RM
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5077-87
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5077-87
Cell activation by ligands of the toll-like receptor and interleukin-1 receptor family depends on the function of the large-conductance potassium channel MaxiK in human macrophages.
Scheel O, Papavlassopoulos M, Blunck R, Gebert A, Hartung T, Zähringer U, Seydel U, Schromm AB
Infection and immunity 2006 Jul;74(7):4354-6
Infection and immunity 2006 Jul;74(7):4354-6
A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression.
Konno K, Wakabayashi Y, Akashi-Takamura S, Ishii T, Kobayashi M, Takahashi K, Kusumoto Y, Saitoh S, Yoshizawa Y, Miyake K
Biochemical and biophysical research communications 2006 Jan 27;339(4):1076-82
Biochemical and biophysical research communications 2006 Jan 27;339(4):1076-82
NF-kappaB- and C/EBPbeta-driven interleukin-1beta gene expression and PAK1-mediated caspase-1 activation play essential roles in interleukin-1beta release from Helicobacter pylori lipopolysaccharide-stimulated macrophages.
Basak C, Pathak SK, Bhattacharyya A, Mandal D, Pathak S, Kundu M
The Journal of biological chemistry 2005 Feb 11;280(6):4279-88
The Journal of biological chemistry 2005 Feb 11;280(6):4279-88
Evidence of Toll-like receptor molecules on human platelets.
Cognasse F, Hamzeh H, Chavarin P, Acquart S, Genin C, Garraud O
Immunology and cell biology 2005 Apr;83(2):196-8
Immunology and cell biology 2005 Apr;83(2):196-8
Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2.
Lotz S, Aga E, Wilde I, van Zandbergen G, Hartung T, Solbach W, Laskay T
Journal of leukocyte biology 2004 Mar;75(3):467-77
Journal of leukocyte biology 2004 Mar;75(3):467-77
Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent.
Mempel M, Voelcker V, Köllisch G, Plank C, Rad R, Gerhard M, Schnopp C, Fraunberger P, Walli AK, Ring J, Abeck D, Ollert M
The Journal of investigative dermatology 2003 Dec;121(6):1389-96
The Journal of investigative dermatology 2003 Dec;121(6):1389-96
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using Azide/BSA FREE version of NB100-56727. Cell surface analysis of TLR4 in stable HEK293/hTLR4 cells using this antibody. Secondary antibody goat anti-mouse IgG-PE at 0.25ug/10^6 cells. Both stable HEK293/hTLR4 and HEK293/Vector cell lines were equally stained.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the PE conjugate of NB100-56727. Staining of TLR4 in human PBMCs using NB100-56062 at 0.5 ug/10^6 cells. The shaded histogram represents cells without anti-TLR4 antibody; green represents the isotype control; red represents Nb100-56062, ant
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the PE conjugate of NB100-56727. Staining of TLR4 on ThP1 cells using TLR4 antibody at 2 ug/10^6 cells. The shaded histogram represents ThP1 cells only, green represents isotype control antibody, and red represents TLR4 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56727. Staining of TLR4 in stable HEK293/hTLR4 cells using this antibody at 1ug/10^6 cells. Green represents HEK293/Vector cells; red represents HEK293/hTLR4 cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56727. Staining of TLR4 on PBMC using this antibody at 1 ug/10^6 cells. Green represents isotype control this antibody; red represents anti-TLR4 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56727. Staining of TLR4 on PBMC using this antibody at 1 ug/10^6 cells. Green represents isotype control this antibody; red represents anti-TLR4 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using Azide/BSA FREE version of NB100-56727. Cell surface analysis of TLR4 in stable HEK293/hTLR4 cells using NB100-56723 at 1 ug/10^6 cells. Secondary antibody Goat anti-Mouse IgG-PE at 0.25ug/10^6 cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using Azide/BSA FREE version of NB100-56727. Analysis of TLR4 using NB100-56723 on PBMCs at 2ug/ 1X10^6 cells. M1 gate are unstained PBMC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (HTA125) - Azide Free [NB100-56727] - Analysis using Azide/BSA FREE version of NB100-56727. Staining of human peripheral blood monocytes with Mouse anti Human CD284.