Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-27149F - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TLR4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. The amino acid sequence used has 100% identity in human, chimp, baboon, chinese hamster, and rat
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (76B357.1) [FITC] [NBP2-27149F] - Analysis using the FITC conjugate of NBP2-27149. Staining of cell surface TLR4 in human monocyte-derived dendritic cells using this antibody at 1:200 (2.5 ug). Black and green open histograms represent unstained cells and negative control (anti-human CD14 FITC, respectively)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (76B357.1) [FITC] [NBP2-27149F] - Analysis using the FITC conjugate of NBP2-27149. TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow test, with CD14 PE and 1 ug of either isotype control (Left, ) or TLR4-FITC (Right, this antibody. PPI negative, CD14+ cells were gated for analysis.