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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [28]
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- Validations
- Flow cytometry [1]
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- Product number
- NB100-56059 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56059, RRID:AB_838999
- Product name
- Mouse Monoclonal TLR4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. The has been shown to block the activation of monocytes with LPS (Paik, et al, 2003).
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
Submitted references Oxidative Stress Induces Expression of the Toll-Like Receptors (TLRs) 2 and 4 in the Human Peripheral Blood Mononuclear Cells: Implications for Metabolic Inflammation.
The Synergy between Palmitate and TNF-α for CCL2 Production Is Dependent on the TRIF/IRF3 Pathway: Implications for Metabolic Inflammation.
Substance P represents a novel first-line defense mechanism in the nose.
Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients.
Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome.
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
TAK-242 (resatorvid), a small-molecule inhibitor of Toll-like receptor (TLR) 4 signaling, binds selectively to TLR4 and interferes with interactions between TLR4 and its adaptor molecules.
TLR4-dependent induction of vascular adhesion molecule-1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A(2)alpha/cyclooxygenase-2.
Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia.
Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes.
Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2.
HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum.
Stimulation of iNOS expression and apoptosis resistance in B-cell chronic lymphocytic leukemia (B-CLL) cells through engagement of Toll-like receptor 7 (TLR-7) and NF-kappaB activation.
Human lymphatic endothelial cells express multiple functional TLRs.
Human lymphatic endothelial cells express multiple functional TLRs.
Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid.
In vitro response to Candida albicans in cultures of whole human blood from young and aged donors.
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Execution of macrophage apoptosis by PE_PGRS33 of Mycobacterium tuberculosis is mediated by Toll-like receptor 2-dependent release of tumor necrosis factor-alpha.
In celiac disease, a subset of autoantibodies against transglutaminase binds toll-like receptor 4 and induces activation of monocytes.
RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes.
RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes.
Cell activation by ligands of the toll-like receptor and interleukin-1 receptor family depends on the function of the large-conductance potassium channel MaxiK in human macrophages.
A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression.
High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production.
Evidence of Toll-like receptor molecules on human platelets.
Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent.
Akhter N, Madhoun A, Arefanian H, Wilson A, Kochumon S, Thomas R, Shenouda S, Al-Mulla F, Ahmad R, Sindhu S
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2019;53(1):1-18
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2019;53(1):1-18
The Synergy between Palmitate and TNF-α for CCL2 Production Is Dependent on the TRIF/IRF3 Pathway: Implications for Metabolic Inflammation.
Ahmad R, Al-Roub A, Kochumon S, Akther N, Thomas R, Kumari M, Koshy MS, Tiss A, Hannun YA, Tuomilehto J, Sindhu S, Rosen ED
Journal of immunology (Baltimore, Md. : 1950) 2018 May 15;200(10):3599-3611
Journal of immunology (Baltimore, Md. : 1950) 2018 May 15;200(10):3599-3611
Substance P represents a novel first-line defense mechanism in the nose.
Larsson O, Tengroth L, Xu Y, Uddman R, Kumlien Georén S, Cardell LO
The Journal of allergy and clinical immunology 2018 Jan;141(1):128-136.e3
The Journal of allergy and clinical immunology 2018 Jan;141(1):128-136.e3
Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients.
Heftrig D, Sturm R, Oppermann E, Kontradowitz K, Jurida K, Schimunek L, Woschek M, Marzi I, Relja B
Mediators of inflammation 2017;2017:2608349
Mediators of inflammation 2017;2017:2608349
Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome.
Jamin A, Dehoux L, Dossier C, Fila M, Heming N, Monteiro RC, Deschênes G
Clinical and experimental immunology 2015 Dec;182(3):332-45
Clinical and experimental immunology 2015 Dec;182(3):332-45
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
Harman AN, Bye CR, Nasr N, Sandgren KJ, Kim M, Mercier SK, Botting RA, Lewin SR, Cunningham AL, Cameron PU
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
TAK-242 (resatorvid), a small-molecule inhibitor of Toll-like receptor (TLR) 4 signaling, binds selectively to TLR4 and interferes with interactions between TLR4 and its adaptor molecules.
Matsunaga N, Tsuchimori N, Matsumoto T, Ii M
Molecular pharmacology 2011 Jan;79(1):34-41
Molecular pharmacology 2011 Jan;79(1):34-41
TLR4-dependent induction of vascular adhesion molecule-1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A(2)alpha/cyclooxygenase-2.
Wu CY, Chi PL, Hsieh HL, Luo SF, Yang CM
Journal of cellular physiology 2010 May;223(2):480-91
Journal of cellular physiology 2010 May;223(2):480-91
Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia.
Pillay J, Ramakers BP, Kamp VM, Loi AL, Lam SW, Hietbrink F, Leenen LP, Tool AT, Pickkers P, Koenderman L
Journal of leukocyte biology 2010 Jul;88(1):211-20
Journal of leukocyte biology 2010 Jul;88(1):211-20
Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes.
Pietschmann K, Beetz S, Welte S, Martens I, Gruen J, Oberg HH, Wesch D, Kabelitz D
Scandinavian journal of immunology 2009 Sep;70(3):245-55
Scandinavian journal of immunology 2009 Sep;70(3):245-55
Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2.
Yang X, Fullerton DA, Su X, Ao L, Cleveland JC Jr, Meng X
Journal of the American College of Cardiology 2009 Feb 10;53(6):491-500
Journal of the American College of Cardiology 2009 Feb 10;53(6):491-500
HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum.
O'Hara SP, Small AJ, Gajdos GB, Badley AD, Chen XM, Larusso NF
The Journal of infectious diseases 2009 Apr 15;199(8):1195-204
The Journal of infectious diseases 2009 Apr 15;199(8):1195-204
Stimulation of iNOS expression and apoptosis resistance in B-cell chronic lymphocytic leukemia (B-CLL) cells through engagement of Toll-like receptor 7 (TLR-7) and NF-kappaB activation.
Hammadi A, Billard C, Faussat AM, Kolb JP
Nitric oxide : biology and chemistry 2008 Sep;19(2):138-45
Nitric oxide : biology and chemistry 2008 Sep;19(2):138-45
Human lymphatic endothelial cells express multiple functional TLRs.
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Human lymphatic endothelial cells express multiple functional TLRs.
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
Burgener IA, Jungi TW
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91
TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid.
Prabha C, Rajashree P, Sulochana DD
Immunology letters 2008 Apr 15;117(1):26-34
Immunology letters 2008 Apr 15;117(1):26-34
In vitro response to Candida albicans in cultures of whole human blood from young and aged donors.
Murciano C, Villamón E, Yáñez A, Murciano J, Mir A, O'Connor JE, Gozalbo D, Gil ML
FEMS immunology and medical microbiology 2007 Nov;51(2):327-35
FEMS immunology and medical microbiology 2007 Nov;51(2):327-35
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
Execution of macrophage apoptosis by PE_PGRS33 of Mycobacterium tuberculosis is mediated by Toll-like receptor 2-dependent release of tumor necrosis factor-alpha.
Basu S, Pathak SK, Banerjee A, Pathak S, Bhattacharyya A, Yang Z, Talarico S, Kundu M, Basu J
The Journal of biological chemistry 2007 Jan 12;282(2):1039-50
The Journal of biological chemistry 2007 Jan 12;282(2):1039-50
In celiac disease, a subset of autoantibodies against transglutaminase binds toll-like receptor 4 and induces activation of monocytes.
Zanoni G, Navone R, Lunardi C, Tridente G, Bason C, Sivori S, Beri R, Dolcino M, Valletta E, Corrocher R, Puccetti A
PLoS medicine 2006 Sep;3(9):e358
PLoS medicine 2006 Sep;3(9):e358
RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes.
Shahrara S, Park CC, Temkin V, Jarvis JW, Volin MV, Pope RM
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5077-87
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5077-87
RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes.
Shahrara S, Park CC, Temkin V, Jarvis JW, Volin MV, Pope RM
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5077-87
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5077-87
Cell activation by ligands of the toll-like receptor and interleukin-1 receptor family depends on the function of the large-conductance potassium channel MaxiK in human macrophages.
Scheel O, Papavlassopoulos M, Blunck R, Gebert A, Hartung T, Zähringer U, Seydel U, Schromm AB
Infection and immunity 2006 Jul;74(7):4354-6
Infection and immunity 2006 Jul;74(7):4354-6
A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression.
Konno K, Wakabayashi Y, Akashi-Takamura S, Ishii T, Kobayashi M, Takahashi K, Kusumoto Y, Saitoh S, Yoshizawa Y, Miyake K
Biochemical and biophysical research communications 2006 Jan 27;339(4):1076-82
Biochemical and biophysical research communications 2006 Jan 27;339(4):1076-82
High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production.
Xu S, Koldovsky U, Xu M, Wang D, Fitzpatrick E, Son G, Koski G, Czerniecki BJ
Surgery 2006 Aug;140(2):170-8
Surgery 2006 Aug;140(2):170-8
Evidence of Toll-like receptor molecules on human platelets.
Cognasse F, Hamzeh H, Chavarin P, Acquart S, Genin C, Garraud O
Immunology and cell biology 2005 Apr;83(2):196-8
Immunology and cell biology 2005 Apr;83(2):196-8
Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent.
Mempel M, Voelcker V, Köllisch G, Plank C, Rad R, Gerhard M, Schnopp C, Fraunberger P, Walli AK, Ring J, Abeck D, Ollert M
The Journal of investigative dermatology 2003 Dec;121(6):1389-96
The Journal of investigative dermatology 2003 Dec;121(6):1389-96
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (HTA125) [FITC] [NB100-56059] - An intracellular stain was performed on MG-63 cells with TLR4 Antibody [HTA125] NB100-56059 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.
