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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NBP1-78427 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-78427, RRID:AB_11018090
- Product name
- Rabbit Polyclonal TLR4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.14 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references TLR4 signaling in VTA dopaminergic neurons regulates impulsivity through tyrosine hydroxylase modulation.
Correlative mRNA and protein expression of middle and inner ear inflammatory cytokines during mouse acute otitis media.
Aurelian L, Warnock KT, Balan I, Puche A, June H
Translational psychiatry 2016 May 17;6(5):e815
Translational psychiatry 2016 May 17;6(5):e815
Correlative mRNA and protein expression of middle and inner ear inflammatory cytokines during mouse acute otitis media.
Trune DR, Kempton B, Hausman FA, Larrain BE, MacArthur CJ
Hearing research 2015 Aug;326:49-58
Hearing research 2015 Aug;326:49-58
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in Raw 264.7 cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in mouse spleen using DAB with hematoxylin counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in mouse smooth muscle using DAB wtih hematoxylin counterstain.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with TLR4 Antibody NBP1-78427AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
