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LearnMA5-16205
antibody from Invitrogen Antibodies
Targeting: TLR4
ARMD10, CD284, hToll, TLR-4
Western blot Immunoprecipitation Immunohistochemistry
Flow cytometry
Blocking/Neutralizing Other assayAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [5]
- Other assay [1]
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- Product number
- MA5-16205 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLR4 Monoclonal Antibody (HTA125)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HTA125
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Elevated Toll-Like Receptor-Induced CXCL8 Secretion in Human Blood Basophils from Allergic Donors Is Independent of Toll-Like Receptor Expression Levels.
The protease inhibitor cystatin C down-regulates the release of IL-β and TNF-α in lipopolysaccharide activated monocytes.
IFN-gamma primes intact human coronary arteries and cultured coronary smooth muscle cells to double-stranded RNA- and self-RNA-induced inflammatory responses by upregulating TLR3 and melanoma differentiation-associated gene 5.
Human CD14 is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes.
Steiner M, Hawranek T, Schneider M, Ferreira F, Horejs-Hoeck J, Harrer A, Himly M
PloS one 2016;11(2):e0149275
PloS one 2016;11(2):e0149275
The protease inhibitor cystatin C down-regulates the release of IL-β and TNF-α in lipopolysaccharide activated monocytes.
Gren ST, Janciauskiene S, Sandeep S, Jonigk D, Kvist PH, Gerwien JG, Håkansson K, Grip O
Journal of leukocyte biology 2016 Oct;100(4):811-822
Journal of leukocyte biology 2016 Oct;100(4):811-822
IFN-gamma primes intact human coronary arteries and cultured coronary smooth muscle cells to double-stranded RNA- and self-RNA-induced inflammatory responses by upregulating TLR3 and melanoma differentiation-associated gene 5.
Ahmad U, Ali R, Lebastchi AH, Qin L, Lo SF, Yakimov AO, Khan SF, Choy JC, Geirsson A, Pober JS, Tellides G
Journal of immunology (Baltimore, Md. : 1950) 2010 Jul 15;185(2):1283-94
Journal of immunology (Baltimore, Md. : 1950) 2010 Jul 15;185(2):1283-94
Human CD14 is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes.
Tunheim G, Schjetne KW, Fredriksen AB, Sandlie I, Bogen B
Journal of leukocyte biology 2005 Mar;77(3):303-10
Journal of leukocyte biology 2005 Mar;77(3):303-10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface flow cytometric analysis of TLR4 in stable HEK293/hTLR4 cells using a TLR4/CD284 monoclonal antibody (Product # MA5-16205) at 1 µg/10^6 cells. Secondary antibody Goat anti-mouse IgG-PE at 0.25 µg/10^6 cells. Both stable HEK293/hTLR4 and HEK293/Vector cell lines were equally stained.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of TLR4 in formaldehyde-fixed THP-1 cells (green) compared to an isotype control (blue). Staining was performed using a TLR4 monoclonal antibody (Product # MA5-16205) at a dilution of 2µg x 10^6 cells., followed by secondary detection using a Donkey anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate (Product # A-21202) at a dilution of 1:200. Mouse IgG2a Isotype Control (Product # MA5-14441) was used as a negative control at the same dilution as the primary antibody. Data provided courtesy of Antibody Resource.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR4 in stable HEK293/hTLR4 cells. Samples were incubated in TLR4 monoclonal antibody (Product # MA5-16205) using a dilution of 0.25 µg/10^6 cells followed by goat anti-mouse IgG-PE secondary antibody. Both stable HEK293/hTLR4 and HEK293/Vector cell lines were equally stained.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR4 in human peripheral blood monocytes with Mouse anti Human CD284. Samples were incubated in TLR4 monoclonal antibody (Product # MA5-16205). Antibody is Azide/BSA FREE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR4 in U937 cells with mouse anti human CD284:RPE. Samples were incubated in TLR4 monoclonal antibody (Product # MA5-16205). Image using the PE form of this antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization validation of TLR4 (CD284) in 1 x 10^5 cells/well TLR4/MD-2/CD14/IL-8 Prom/LUCPorter HeLa cells. Samples were incubated in TLR4 (CD284) monoclonal antibody (Product # MA5-16205). After 16 h, cells were preincubated with different amounts of this antibody for 1 h and then stimulated with 10 ng/mL LPS for 6 h. Luciferase activity was then directly analyzed using the one-step luciferase reporter assay reagent. Data Summary: It inhibited LPS-mediated TLR4 activity in the TLR4/MD-2/CD14/IL-8 Prom/LUCPorter cell line. Image using the Azide Free format of this antibody.
