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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- 101-M32 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- VEGFR-2/KDR
- Antibody type
- Monoclonal
- Antigen
- recombinant human soluble KDR (D1-7)
- Description
- antibody purified from hybridoma supernatant
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
ASVGLPSVSLDLPRLSIQKDILTIKANTTLQITCR
GQRDLDWLWPNNQSGSEQRVEVTECSDGLFCKTLT
IPKVIGNDTGAYKCFYRETDLASVIYVYVQDYRSP
FIASVSDQHGVVYITENKNKTVVIPCLGSISNLNV
SLCARYPEKRFVPDGNRISWDSKKGFTIPSYMISY
AGMVFCEAKINDESYQSIMYIVVVVGYRIYDVVLS
PSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPS
SKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTR
SDQGLYTCAASSGLMTKKNSTFVRVHEKPFVAFGS
GMESLVEATVGERVRIPAKYLGYPPPEIKWYKNGI
PLESNHTIKAGHVLTIMEVSERDTGNYTVILTNPI
SKEKQSHVVSLVVYVPPQIGEKSLISPVDSYQYGT
TQTLTCTVYAIPPPHHIHWYWQLEEECANEPSQAV
SVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEG
KNKTVSTLVIQAANVSALYKCEAVNKVGRGERVIS
FHVTRGPEITLQPDMQPTEQESVSLWCTADRSTFE
NLTWYKLGPQPLPIHVGELPTPVCKNLDTLWKLNA
TMFSNSTNDILIMELKNASLQDQGDYVCLAQDRKT
KKRHCVVRQLTVLERVAPTITGNLENQTTSIGESI
EVSCTASGNPPPQIMWFKDNETLVEDSGIVLKDGN
RNLTIRRVRKEDEGLYTCQACSVLGCAKVEAFFII
EGA- Isotype
- IgG
- Antibody clone number
- (#3(4H3))
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references DNA methylation regulates expression of VEGF-R2 (KDR) and VEGF-R3 (FLT4)
Expression of Vascular Endothelial Growth Factor Receptor-2 or Tie-2 on Peripheral Blood Cells Defines Functionally Competent Cell Populations Capable of Reendothelialization
Elevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas
Quentmeier H, Eberth S, Romani J, Weich H, Zaborski M, Drexler H
BMC Cancer 2012 ;12(1):19
BMC Cancer 2012 ;12(1):19
Expression of Vascular Endothelial Growth Factor Receptor-2 or Tie-2 on Peripheral Blood Cells Defines Functionally Competent Cell Populations Capable of Reendothelialization
Nowak G
Circulation 2004 December;110(24):3699-3707
Circulation 2004 December;110(24):3699-3707
Elevated expression of VEGFR-3 in lymphatic endothelial cells from lymphangiomas
Norgall S, Papoutsi M, Rössler J, Schweigerer L, Wilting J, Weich H
BMC Cancer ;7(1):105
BMC Cancer ;7(1):105
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- VEGFR-2/KDR Sandwich-ELISA using soluble KDR (D7) [Cat# S01-002] as standard. Mouse anti-human VEGFR-2 [Cat# 101-M32] was used as capture antibody, Biotinylated rabbit anti-human VEGFR-2 [Cat# 102-PABi18] was used for detection.
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Up-regulation of VEGFR-2 in primary HUVECs by bFGF. Freshly isolated HUVECs (passage 1) were cultured in EBM. Subconfluent cultures were stimulated with VEGF (5 ng/ml) or bFGF (10 ng/ml) for 3 days. Total lysate was prepared and subjected to immunoprecipitation (anti-human VEGFR-2 (Cl.3) [#101-M32] followed by Western blotting (anti-human VEGFR-2 (Cl.4) [#101-M34].
- Sample type
- HUVEC
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining with consecutive sections of unfixed, human foreskin. A) Staining with anti-sVEGFR2/KDR antibodies (#102-PA19). Note signal in epidermis and vessels. B) Staining with anti-membrane-bound VEGFR-2/KDR (#101-M32). Note staining in vessels. C) Negative control. Note non-specific fluorescence in the hornified layer of the epithelium. Provided by Prof. J. Wilting, Göttingen, Germany.
- Sample type
- Human Foreskin
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis of VEGFR-2/KDR expression in HUVE cells [5µg/ml #101-M32; 5µg/ml PE goat anti-mouse IgG]. The experiment was performed by Trisha M. Westerhof, University of California, Irvine.
- Sample type
- HUVEC
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with HDMVEC and HUVEC.
- Sample type
- HDMVEC and HUVEC
