ABIN1504513

antibody from antibodies-online

Targeting: KDR CD309, FLK1, VEGFR, VEGFR2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN1504513

Provider

antibodies-online

Product name

anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) antibody

Antibody type

Polyclonal

Antigen

Other

Description

Affinity Chromatography

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.2 mL

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis (Representative lot data): PAE cells transfected with CSF/VEGFR-2 chimera and treated with CSF was probed with Anti-KDR/Flk-1/VEGFR-2 (1/1000 dilution). Proteins were visualized using a donkey anti rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates KDR/Flk-1/VEGFR-2 (~150/180 kDa).

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Immunohistochemistry analysis (Representative lot data): Paraffin-embedded mouse embryo vascular endothelium tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1/100 dilution of Anti-KDR/Flk-1/VEGFR-2. Reactivity was detected using an IHC-Detection Kit. Staining pattern appears to be restricted endothelial cells with no smooth muscle involvement.